Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Luis Mario Leal Garza & Luis Fernando Camarillo Guerrero
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:33
HlyA-tag+Secretion system
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1166002_sequence (Version 1) |
Description
This secretion device includes all the necessary elements (linker-HlyA secretion signal peptide and the expression cassette for HlyB and HlyD) for the secretion of expression cassettes cloned in the 5??? terminal of the device.This secretion device is usable by Silver standard fusion in its 5??? end for proteins with no stop codon.
Codes for both the proteins HlyB and HlyD (native from uropathogenic strains of E.coli), which, along with the constitutively expressed TolC protein, form a secretion channel. This secretion system recognizes proteins tagged with the C-terminal secretion signal peptide HlyA, and secretes them to the exterior of the bacteria.
Notes
HlyA C terminal part has a 5' section compatible with Silver Fusion standard. Downstream, HlyB and HlyD are both under the AraC/pBAD promoter to be induced by arabinose.Source
The HlyB and HlyD sequences come from the parts registry, while the HlyA from the registry had mayor problems, so we took it from the genomic sequence.| Sequence Annotation | Location | Component / Role(s) |
| Linker HlyA stop BBa_B0015 AraC+pBAD BBa_B0034 spacer HlyB stop BBa_B0034 spacer HlyD stop BBa_B0015 | 1,30 31,210 211,216 217,345 346,1555 1556,1567 1568,1573 1574,3694 3695,3700 3701,3712 3713,3718 3719,5152 5153,5158 5159,5287 | feature/misc sequence_feature feature/tag tag feature/stop stop_codon feature/stem_loop stem_loop promoter feature/promoter ribosome_entry_site feature/rbs sequence_feature feature/misc CDS feature/cds feature/stop stop_codon feature/rbs ribosome_entry_site feature/misc sequence_feature CDS feature/cds stop_codon feature/stop stem_loop feature/stem_loop |