LOCUS BBa_K1166002 5287 bp DNA linear 24-Apr-2024 DEFINITION HlyA-tag+Secretion system ACCESSION BBa_K1166002 VERSION BBa_K1166002.1 FEATURES Location/Qualifiers misc_feature 1..30 /label=Linker misc_feature 31..210 /label=HlyA misc_feature 211..216 /label=stop stem_loop 217..345 /label=BBa_B0015 promoter 346..1555 /label=AraC+pBAD RBS 1556..1567 /label=BBa_B0034 misc_feature 1568..1573 /label=spacer CDS 1574..3694 /label=HlyB misc_feature 3695..3700 /label=stop RBS 3701..3712 /label=BBa_B0034 misc_feature 3713..3718 /label=spacer CDS 3719..5152 /label=HlyD misc_feature 5153..5158 /label=stop stem_loop 5159..5287 /label=BBa_B0015 ORIGIN 1 ggcggtggcg gtagcggcgg tggcggttct ttagcctatg gaagtcaggg tgatcttaat 61 ccattaatta atgaaatcag caaaatcatt tcagcagcag gtagcttcga tgttaaagag 121 gaaagaaccg cagcttcttt attgcagttg tccggtaatg ccagtgattt ttcatatgga 181 cggaactcaa taaccctgac cacatcagca taataaccag gcatcaaata aaacgaaagg 241 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 301 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatattatg acaacttgac 361 ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt 421 gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac 481 ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc 541 ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga 601 cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg 661 atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc 721 gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc 781 cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg 841 cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt 901 aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg 961 agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc 1021 cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag 1081 attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt 1141 ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag 1201 gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa 1261 ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac 1321 caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac 1381 aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca 1441 cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg 1501 acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaaaga 1561 ggagaaatac tagatggact cctgtcacaa gatcgactac ggcctgtatg cactggaaat 1621 cctggctcaa taccacaacg tgtcggtcaa cccggaagaa attaaacacc gctttgacac 1681 ggatggtacc ggcctgggtc tgacgtcgtg gctgctggcc gcaaagagcc tggaactgaa 1741 agtgaaacag gtgaagaaga cgattgaccg cctgaacttt atcagcctgc cagctctggt 1801 ttggcgcgaa gatggtcgtc atttcatcct gacgaaagtg tcgaaggaag caaatcgcta 1861 tctgatcttc gacctggaac agcgcaatcc gcgtgtgctg gaacaaagcg aatttgaagc 1921 gctgtaccaa ggtcacatca ttctgattgc ctcacgctcc agcgttacgg gcaaactggc 1981 caagttcgat tttacctggt ttattccggc catcattaaa tatcgtaaga ttttcatcga 2041 aaccctggtc gtgagcgtgt ttctgcaact gttcgcactg attacgccgc tgtttttcca 2101 ggtggtcatg gataaggtgc tggtccatcg tggtttttcg accctgaacg tgatcacggt 2161 tgctctgtca gttgtggtgg tgtttgaaat tattctgagc ggtctgcgta cctacatctt 2221 tgcacactcg acctctcgta tcgacgttga actgggtgcg aagctgttcc gccacctgct 2281 ggcactgccg attagctact ttgaaagccg tcgtgttggt gataccgtgg cccgcgtgcg 2341 tgaactggac caaattcgta attttctgac cggccaggcc ctgacgtccg tcctggatct 2401 gctgttctcg tttattttct ttgccgttat gtggtattac agtccgaagc tgacgctggt 2461 gattctgttt agtctgccgt gctatgcggc atggagcgtt tttatcagcc cgattctgcg 2521 tcgtcgcctg gatgacaaat ttagccgtaa tgccgacaac cagtcatttc tggtcgaatc 2581 cgttaccgca attaatacca tcaaggctat ggcagtttca ccgcaaatga ccaacatttg 2641 ggataaacaa ctggcaggtt acgttgcagc aggtttcaag gtgaccgttc tggctaccat 2701 tggccagcag ggtattcaac tgatccagaa aacggtgatg atcatcaacc tgtggctggg 2761 tgctcatctg gtgatttctg gcgatctgag cattggtcaa ctgattgcct tcaatatgct 2821 ggcgggccag atcgtggccc cggtgatccg tctggcacag atttggcaag acttccaaca 2881 ggtcggtatt agtgtgacgc gtctgggcga cgtgctgaac agcccgaccg aatcctatca 2941 tggcaaactg gctctgccgg aaatcaatgg taacattacg ttccgcaata ttcgtttccg 3001 ctacaaaccg gattcgccgg tgattctgga taacatcaac ctgtctatca aacagggtga 3061 agtgattggt attgtgggtc gctccggttc aggtaagagt acgctgacca aactgatcca 3121 gcgtttttat atcccggaaa acggtcaagt cctgattgac ggccatgatc tggcactggc 3181 ggacccgaac tggctgcgcc gtcaagtggg tgtggtgctg caagacaatg tgctgctgaa 3241 ccgcagcatt attgacaata tcagtctggc aaatccgggc atgtcggtgg aaaaggtcat 3301 ctacgctgcc aagctggcag gcgcccacga tttcattagc gaactgcgcg aaggctataa 3361 cacgattgtg ggcgaacagg gtgcaggcct gtctggcggt caacgtcagc gcattgcaat 3421 tgctcgcgcg ctggtcaaca atccgaaaat tctgattttt gacgaagcga ccagtgccct 3481 ggattatgaa agcgaacata ttatcatgcg taacatgcac aaaatctgca agggccgcac 3541 ggtcattatc attgcgcacc gtctgagtac cgtcaagaac gcggatcgta tcattgtgat 3601 ggaaaagggt aaaatcgtgg aacaaggcaa gcataaggaa ctgctgtcgg aaccggaaag 3661 cctgtactcg tatctgtatc aactgcaatc ggattaataa aaagaggaga aatactagat 3721 gaagacgtgg ctgatgggct ttagtgaatt tctgctgcgt tacaaactgg tgtggtcgga 3781 aacctggaag attcgcaaac aactggatac cccggtgcgc gaaaaagacg aaaacgaatt 3841 tctgccagcg catctggaac tgattgaaac gccggtcagt cgtcgcccgc gtctggtggc 3901 gtattttatc atgggcttcc tggttattgc cgtcatcctg tccgttctgg gtcaggtgga 3961 aattgttgca accgctaacg gcaaactgac gctgagcggt cgctctaaag aaattaagcc 4021 gatcgaaaat agcatcgtca aagaaattat cgtgaaggaa ggcgaaagcg tgcgtaaagg 4081 tgatgtcctg ctgaagctga ccgccctggg cgcagaagca gacaccctga aaacgcagag 4141 ctctctgctg caaacccgtc tggaacagac gcgctatcaa attctgtcac gctcgatcga 4201 actgaacaaa ctgccggaac tgaagctgcc ggatgaaccg tactttcaga atgtgagcga 4261 agaagaagtg ctgcgtctga ccagcctgat taaagaacag ttctctacgt ggcagaacca 4321 aaagtaccaa aaggaactga atctggataa aaagcgtgca gaacgcctga ccattctggc 4381 tcgtatcaac cgctacgaaa atctgtcacg tgtggaaaaa tcgcgcctgg atgactttcg 4441 tagtctgctg cataaacagg caattgctaa gcacgcagtt ctggaacaag aaaacaaata 4501 tgtcgaagcg gccaatgaac tgcgcgtgta caagagccaa ctggaacaaa ttgaaagtga 4561 aatcctgtcc gccaaagaag aatatcaact ggtgacccaa ctgttcaaaa atgaaattct 4621 ggataagctg cgtcagacca cggacaacat cgaactgctg accctggaac tggaaaagaa 4681 cgaagaacgt cagcaagcaa gtgtgattcg tgctccggtt tccggtaaag tccagcaact 4741 gaaggttcat accgaaggcg gtgtggttac cacggcagaa acgctgatgg tcatcgtgcc 4801 ggaagatgac accctggaag tgacggctct ggttcagaac aaagatattg gctttatcaa 4861 cgttggtcag aatgcgatta tcaaggtcga agccttcccg tacacccgct atggctacct 4921 ggtcggtaaa gtgaagaaca ttaatctgga tgcgatcgaa gaccagaaac tgggcctggt 4981 gttcaatgtt attgtctcag tggaagaaaa cgatctgtcg accggcaata aacacattcc 5041 gctgagttcc ggtatggcgg ttaccgccga aatcaaaacg ggtatgcgtt ctgtcatctc 5101 ctatctgctg tcgccgctgg aagaatctgt cacggaatcg ctgcatgaac gttaataacc 5161 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 5221 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 5281 gtttata //