LOCUS       BBa_K116633              658 bp    DNA     linear       10-Jun-2024
DEFINITION  pLac + RBS + CII + T
ACCESSION   BBa_K116633
VERSION     BBa_K116633.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     CDS             228..521
                     /label=BBa_K116602
     stem_loop       541..584
                     /label=stem_loop
     misc_feature    530..609
                     /label=BBa_B0010
     terminator      530..609
                     /label=BBa_B0010
     stem_loop       625..644
                     /label=T7 TE
     misc_feature    651..651
                     /label=stop
     polyA_site      645..658
                     /label=polya
     misc_feature    618..658
                     /label=BBa_B0012
     terminator      618..658
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg gttcgtgcaa
      241 acaaacgcaa cgaggctcta cgaatcgaga gtgcgttgct taacaaaatc gcaatgcttg
      301 gaactgagaa gacagcggaa gctgtgggcg ttgataagtc gcagatcagc aggtggaaga
      361 gggactggat tccaaagttc tcaatgctgc ttgctgttct tgaatggggg gtcgttgacg
      421 acgacatggc tcgattggcg cgacaagttg ctgcgattct caccaataaa aaacgcccgg
      481 cggcaaccga gcgttctgaa caaatccaga tggagttctg atactagagc caggcatcaa
      541 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      601 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//