LOCUS       BBa_K116643             1598 bp    DNA     linear       09-Jun-2024
DEFINITION  pRE tester
ACCESSION   BBa_K116643
VERSION     BBa_K116643.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     CDS             228..521
                     /label=BBa_K116602
     stem_loop       541..584
                     /label=stem_loop
     misc_feature    530..609
                     /label=BBa_B0010
     terminator      530..609
                     /label=BBa_B0010
     stem_loop       625..644
                     /label=T7 TE
     misc_feature    651..651
                     /label=stop
     polyA_site      645..658
                     /label=polya
     misc_feature    618..658
                     /label=BBa_B0012
     terminator      618..658
                     /label=BBa_B0012
     promoter        667..714
                     /label=BBa_K116603
     RBS             723..735
                     /label=RBS-3\Weak
     RBS             729..732
                     /label=RBS
     misc_feature    723..735
                     /label=BBa_B0032
     RBS             723..735
                     /label=BBa_B0032
     CDS             742..1461
                     /label=GFP protein
     CDS             742..1461
                     /label=BBa_E0040
     stem_loop       1481..1524
                     /label=stem_loop
     misc_feature    1470..1549
                     /label=BBa_B0010
     terminator      1470..1549
                     /label=BBa_B0010
     stem_loop       1565..1584
                     /label=T7 TE
     misc_feature    1591..1591
                     /label=stop
     polyA_site      1585..1598
                     /label=polya
     misc_feature    1558..1598
                     /label=BBa_B0012
     terminator      1558..1598
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg gttcgtgcaa
      241 acaaacgcaa cgaggctcta cgaatcgaga gtgcgttgct taacaaaatc gcaatgcttg
      301 gaactgagaa gacagcggaa gctgtgggcg ttgataagtc gcagatcagc aggtggaaga
      361 gggactggat tccaaagttc tcaatgctgc ttgctgttct tgaatggggg gtcgttgacg
      421 acgacatggc tcgattggcg cgacaagttg ctgcgattct caccaataaa aaacgcccgg
      481 cggcaaccga gcgttctgaa caaatccaga tggagttctg atactagagc caggcatcaa
      541 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      601 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata
      661 ctagagagag cctcgttgcg tttgtttgca cgaaccatat gtaagtattt cctttactag
      721 agtcacacag gaaagtacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
      781 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      841 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      901 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      961 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
     1021 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
     1081 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
     1141 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
     1201 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
     1261 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
     1321 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
     1381 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     1441 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc
     1501 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1561 cactggctca ccttcgggtg ggcctttctg cgtttata
//