LOCUS       BBa_K116665             2184 bp    DNA     linear       09-Jun-2024
DEFINITION  Short Synchronizer
ACCESSION   BBa_K116665
VERSION     BBa_K116665.1  
FEATURES             Location/Qualifiers
     promoter        1..48
                     /label=BBa_K116603
     RBS             57..69
                     /label=RBS-3\Weak
     RBS             63..66
                     /label=RBS
     misc_feature    57..69
                     /label=BBa_B0032
     RBS             57..69
                     /label=BBa_B0032
     misc_feature    76..77
                     /label=prefix
     misc_feature    249..249
                     /label=T
     misc_feature    567..567
                     /label=A
     CDS             76..825
                     /label=luxR
     misc_feature    76..831
                     /label=BBa_C0062
     misc_feature    832..856
                     /label=Help:Barcodes
     CDS             76..831
                     /label=BBa_C0062
     misc_feature    57..856
                     /label=BBa_K116616
     RBS             865..877
                     /label=RBS-3\Weak
     RBS             871..874
                     /label=RBS
     misc_feature    865..877
                     /label=BBa_B0032
     RBS             865..877
                     /label=BBa_B0032
     misc_feature    1463..1494
                     /label=LVA
     CDS             884..1462
                     /label=luxI
     misc_feature    884..1501
                     /label=BBa_C0061
     misc_feature    1502..1526
                     /label=Help:Barcodes
     CDS             884..1501
                     /label=BBa_C0061
     stem_loop       1546..1589
                     /label=stem_loop
     misc_feature    1535..1614
                     /label=BBa_B0010
     terminator      1535..1614
                     /label=BBa_B0010
     stem_loop       1630..1649
                     /label=T7 TE
     misc_feature    1656..1656
                     /label=stop
     polyA_site      1650..1663
                     /label=polya
     misc_feature    1623..1663
                     /label=BBa_B0012
     terminator      1623..1663
                     /label=BBa_B0012
     terminator      1535..1663
                     /label=BBa_B0015
     misc_feature    884..1663
                     /label=BBa_K116615
     misc_feature    865..1663
                     /label=BBa_K116625
     misc_feature    57..1663
                     /label=BBa_K116634
     misc_feature    1672..1691
                     /label=LuxR/HSL
     promoter        1691..1696
                     /label=-35
     promoter        1713..1718
                     /label=-10
     misc_feature    1724..1724
                     /label=start
     misc_feature    1672..1726
                     /label=BBa_R0062
     promoter        1672..1726
                     /label=BBa_R0062
     misc_feature    57..1726
                     /label=BBa_K116645
     misc_feature    1..1726
                     /label=BBa_K116655
     RBS             1735..1747
                     /label=RBS-3\Weak
     RBS             1741..1744
                     /label=RBS
     misc_feature    1735..1747
                     /label=BBa_B0032
     RBS             1735..1747
                     /label=BBa_B0032
     CDS             1754..2047
                     /label=BBa_K116602
     stem_loop       2067..2110
                     /label=stem_loop
     misc_feature    2056..2135
                     /label=BBa_B0010
     terminator      2056..2135
                     /label=BBa_B0010
     stem_loop       2151..2170
                     /label=T7 TE
     misc_feature    2177..2177
                     /label=stop
     polyA_site      2171..2184
                     /label=polya
     misc_feature    2144..2184
                     /label=BBa_B0012
     terminator      2144..2184
                     /label=BBa_B0012
     misc_feature    1735..2184
                     /label=BBa_K116622
ORIGIN
        1 agagcctcgt tgcgtttgtt tgcacgaacc atatgtaagt atttccttta ctagagtcac
       61 acaggaaagt actagatgaa aaacataaat gccgacgaca catacagaat aattaataaa
      121 attaaagctt gtagaagcaa taatgatatt aatcaatgct tatctgatat gactaaaatg
      181 gtacattgtg aatattattt actcgcgatc atttatcctc attctatggt taaatctgat
      241 atttcaatcc tagataatta ccctaaaaaa tggaggcaat attatgatga cgctaattta
      301 ataaaatatg atcctatagt agattattct aactccaatc attcaccaat taattggaat
      361 atatttgaaa acaatgctgt aaataaaaaa tctccaaatg taattaaaga agcgaaaaca
      421 tcaggtctta tcactgggtt tagtttccct attcatacgg ctaacaatgg cttcggaatg
      481 cttagttttg cacattcaga aaaagacaac tatatagata gtttattttt acatgcgtgt
      541 atgaacatac cattaattgt tccttctcta gttgataatt atcgaaaaat aaatatagca
      601 aataataaat caaacaacga tttaaccaaa agagaaaaag aatgtttagc gtgggcatgc
      661 gaaggaaaaa gctcttggga tatttcaaaa atattaggtt gcagtgagcg tactgtcact
      721 ttccatttaa ccaatgcgca aatgaaactc aatacaacaa accgctgcca aagtatttct
      781 aaagcaattt taacaggagc aattgattgc ccatacttta aaaattaata acactgatag
      841 tgctagtgta gatcactact agagtcacac aggaaagtac tagatgacta taatgataaa
      901 aaaatcggat tttttggcaa ttccatcgga ggagtataaa ggtattctaa gtcttcgtta
      961 tcaagtgttt aagcaaagac ttgagtggga cttagttgta gaaaataacc ttgaatcaga
     1021 tgagtatgat aactcaaatg cagaatatat ttatgcttgt gatgatactg aaaatgtaag
     1081 tggatgctgg cgtttattac ctacaacagg tgattatatg ctgaaaagtg tttttcctga
     1141 attgcttggt caacagagtg ctcccaaaga tcctaatata gtcgaattaa gtcgttttgc
     1201 tgtaggtaaa aatagctcaa agataaataa ctctgctagt gaaattacaa tgaaactatt
     1261 tgaagctata tataaacacg ctgttagtca aggtattaca gaatatgtaa cagtaacatc
     1321 aacagcaata gagcgatttt taaagcgtat taaagttcct tgtcatcgta ttggagacaa
     1381 agaaattcat gtattaggtg atactaaatc ggttgtattg tctatgccta ttaatgaaca
     1441 gtttaaaaaa gcagtcttaa atgctgcaaa cgacgaaaac tacgctttag tagcttaata
     1501 actctgatag tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct
     1561 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1621 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gacctgtagg
     1681 atcgtacagg tttacgcaag aaaatggttt gttatagtcg aataaatact agagtcacac
     1741 aggaaagtac tagatggttc gtgcaaacaa acgcaacgag gctctacgaa tcgagagtgc
     1801 gttgcttaac aaaatcgcaa tgcttggaac tgagaagaca gcggaagctg tgggcgttga
     1861 taagtcgcag atcagcaggt ggaagaggga ctggattcca aagttctcaa tgctgcttgc
     1921 tgttcttgaa tggggggtcg ttgacgacga catggctcga ttggcgcgac aagttgctgc
     1981 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
     2041 gttctgatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     2101 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     2161 cgggtgggcc tttctgcgtt tata
//