Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nguyen Xuan Hung
Date created: 2008-10-06 11:00:00
Date modified: 2015-05-08 01:09:34
LsrA promoter (indirectly activated by AI-2)
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K117002_sequence (Version 1) |
Description
The regulatory network for the uptake of Escherichia coli autoinducer 2 (AI-2) is comprised of a transporter complex, LsrABCD; its repressor, LsrR; and a cognate signal kinase, LsrK. This network is an integral part of the AI-2 quorum-sensing (QS) system.Once uptaken into the cell, AI-2 will be phosphorylated under the effect of LsrK. Without the presence of AI-2, LsrA promoter is inhibited by the repressor, LsrR. Phosphorylated AI-2 will prevent LsrR from inhibiting this promoter, hence activating it so that the downstream sequence can be expressed.
To prevent the cell from self-inducing this promoter, we must suppress its AI-2 producing capability. It can be done by knocking out LuxS, one of the vital gene required for producing AI-2. By doing that, LsrA promoter can only be activated under the presence of AI-2 from other sources than the host cell which is LuxS mutant (say, by contact with normal cells).
More information on AI-2 quorum-sensing (QS) system please refer to:
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1952038
Notes
to be updatedSource
to be updated| Sequence Annotation | Location | Component / Role(s) |
| RBS BBa_B0034 | 112,123 | ribosome_entry_site feature/rbs |