LOCUS       BBa_K117007             2036 bp    DNA     linear       18-May-2024
DEFINITION  colE7ImmE7-double terminator BBa_B0015
ACCESSION   BBa_K117007
VERSION     BBa_K117007.1  
FEATURES             Location/Qualifiers
     RBS             117..124
                     /label=caeE7 RBS
     misc_feature    478..587
                     /label=caeE7 toxicity domain
     CDS             1862..1899
                     /label=Immunity (ceiE7)
     misc_feature    1..1899
                     /label=BBa_K117001
     stem_loop       1919..1962
                     /label=stem_loop
     misc_feature    1908..1987
                     /label=BBa_B0010
     terminator      1908..1987
                     /label=BBa_B0010
     stem_loop       2003..2022
                     /label=T7 TE
     misc_feature    2029..2029
                     /label=stop
     polyA_site      2023..2036
                     /label=polya
     misc_feature    1996..2036
                     /label=BBa_B0012
     terminator      1996..2036
                     /label=BBa_B0012
ORIGIN
        1 aatatccctc cctgacttga cagagaaaat aatggcgagt actttttgat ctgtacataa
       61 aaccagtggt tttatgtaca gtattaacta tgtaattaat tgttttaacg cttaaaagag
      121 ggaattttta tgagcggtgg agatggacgt ggccacaaca gtggcgcaca taacacaggt
      181 ggtaacatta atggcggccc tacggggctt ggtggaaatg gtggggcttc tgacggctcc
      241 ggatggagtt cggaaaataa cccatggggt ggcggttccg gtagtggtgt tcactgggga
      301 ggtggctccg gccatggcaa tggcgggggg aatagtaatt ccggtggtgg cagcaattca
      361 tccgtagcag cacctatggc atttggtttt cctgctttgg ctgctcctgg tgccggaact
      421 cttggtattt ccgtatctgg tgaggcttta tctgcggcaa tagcggatat ctttgcagcc
      481 ctgaaaggtc cgtttaaatt cagcgcatgg ggtattgcgc tttacggcat tctgccatct
      541 gaaatagcaa aagatgaccc gaatatgatg tcaaagattg tgacgtcatt accggcagaa
      601 acagtgacga atgttcaggt cagtacactt ccgctggacc aggcaactgt cagcgtgacg
      661 aaacgtgtga cagatgttgt gaaggacaca cgacagcata ttgccgtagt tgcaggtgtg
      721 cctatgagtg tgcctgttgt gaatgccaaa ccaacacgta ctccaggtgt atttcacgca
      781 tcatttcctg gtgtcccttc tctgacagtc agcactgtca aaggccttcc ggtgtcaaca
      841 acccttcccc gtggtattac ggaggataaa ggccggactg ccgttcctgc gggatttacc
      901 tttggtggtg gttcacatga agcggtgatt cgctttccga aagaaagtgg gcagaagccg
      961 gtttatgtat cagtgacaga tgttcttacc ccagcacagg taaaacaacg tcaggatgag
     1021 gaaaaacgcc tccagcagga atggaatgac gcacatccgg tggaagtggc tgaacgcaat
     1081 tatgaacagg cccgcgcaga actgaatcag gctaataaag atgttgccag aaaccaggaa
     1141 cgacaggcta aagctgttca ggtttataat tctcgtaaaa gtgaacttga tgcagcgaat
     1201 aaaactcttg ctgatgcaaa ggctgaaata aaacaattcg agcgatttgc ccgagaacca
     1261 atggctgctg gtcacagaat gtggcaaatg gcagggctta aggcccagcg ggcacagacg
     1321 gatgtaaata ataagaaggc tgcatttgat gctgctgcaa aagaaaagtc agatgctgat
     1381 gttgcattga gttctgcgtt ggagcgccgc aaacagaaag aaaataaaga aaaggacgct
     1441 aaagccaaat tagataagga gagtaaacgg aataagccag ggaaggcaac aggtaaagga
     1501 aaacctgtca ataataagtg gttaaataat gcaggtaaag acttaggttc tcctgttcca
     1561 gatcgtatag ctaataaact acgtgataag gagtttaaaa gtttcgatga ttttcgtaag
     1621 aaattctggg aagaagtgtc aaaagatcct gagttaagta aacaatttag taggaacaat
     1681 aatgatcgaa tgaaggttgg aaaagcgccc aagactagaa cccaggatgt ttcagggaag
     1741 agaacttcat tcgagcttca tcatgagaag ccgatcagcc aaaatggtgg tgtctatgat
     1801 atggataaca tcagcgtggt aacacctaaa aggcatattg atattcaccg aggtaaataa
     1861 tatggaactg aaaaatagta ttagtgatta cacagaggct actagagcca ggcatcaaat
     1921 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1981 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//