LOCUS       BBa_K1172566            2446 bp    DNA     linear       23-May-2024
DEFINITION  Combination of oprF under control of T7 promoter and gldA coding 
            sequence
ACCESSION   BBa_K1172566
VERSION     BBa_K1172566.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     CDS             39..1336
                     /label=cds
     misc_feature    39..41
                     /label=start
     misc_feature    1334..1336
                     /label=stop
     CDS             39..1336
                     /label=BBa_K1172501
     misc_feature    1..1336
                     /label=BBa_K1172502
     CDS             1343..2446
                     /label=gldA
     misc_feature    1343..1345
                     /label=start
     misc_feature    2444..2446
                     /label=stop
     CDS             1343..2446
                     /label=BBa_K1172201
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat gaaactgaaa aacaccttgg
       61 gctttgcaca ccggggaagt cggcaaacag attgttgaaa ctgcggtttt cgccgtagat
      121 gaccaccaca ttcttcacgt tatcgtgcag ggccttgtcc agctcagctg gcgacagttc
      181 gcgggtgact accggcccag gcggttcagc gggcccaccg caggcgctca gggtcgcccc
      241 ggcgcccagt acggcaaccc ctcctaaaaa gcgccgacgg cttgaatcgg ggagcgtatc
      301 ggcggcattt tgggtggctt tcgggtcttc ggtttcttcg gtcatccgag gcatctcacg
      361 gctgtttttg gtttatttgt tgctggatgt ttcgttgaaa aggcatcaaa tatggcaaag
      421 cttaaacgct tcagtctata aggcgaggcc ttagggcata agggttctga tgatgactat
      481 cgggtttcgt aacgtggccg cgctgcaatg caccctgtgc cccgtgatcg attgacggtc
      541 gatcaagggg ccagaggcgg tgccgggcct gagggcgaag cttaagcagg caatgtgaca
      601 gaagcgttta cggcatcacg ggaggcaggt atttgagcgt ggtgcctagc gcccaaagca
      661 ggaacaacac caacggcgtg tgcaccagca gttgcacaaa cgagaaacca atcaggtctc
      721 gtgccttcag ccccaacacg ccgagcaacg gcagcatgta gaacgggttg atcaagttcg
      781 gcagcgcctc ggcagcgttg tagatctgta ccgcccagcc caagtggtat tgcagctcat
      841 tggccacttg catcacgtaa ggcgcttcga tgatccactt gccgcccccc gatggaataa
      901 agaaacccag caccgccgag tacacgccca tcaacaacgc ataggtgtcg tgagaggcga
      961 tggaggtaaa gaaggtcgag atatggtgcg ccagggtctg gccgtcgcca cccttgacca
     1021 cggtcatcag tgcagcgatc gagccataca gcggaaactg gatcaacacg ccggtggtgg
     1081 tcggcacggc acgcgccacc gcatcgagga aactgcgcgg gcgccagtgc agcagtgcgc
     1141 caaccatgat gaacaggaag ttataggtgt tcagccctga aatggcgctg atcgccggct
     1201 tggtcgaaaa ctcatggaat aaccaacctg ccgccagcaa tgcaagcaga atggtcagca
     1261 gcgggctgtg ctccaaccat tcgcccggac ggcttggcgc ttgaatcttg cgcaggttga
     1321 agcccaagct aagtaatact agatggaccg cattattcaa tcaccgggta aatacatcca
     1381 gggcgctgat gtgattaatc gtctgggcga atacctgaag ccgctggcag aacgctggtt
     1441 agtggtgggt gacaaatttg ttttaggttt tgctcaatcc actgtcgaga aaagctttaa
     1501 agatgctgga ctggtagtag aaattgcgcc gtttggcggt gaatgttcgc aaaatgagat
     1561 cgaccgtctg cgtggcatcg cggagactgc gcagtgtggc gcaattctcg gtatcggtgg
     1621 cggaaaaacc ctcgatactg ccaaagcact ggcacatttc atgggtgttc cggtagcgat
     1681 cgcaccgact atcgcctcta ccgatgcacc gtgcagcgca ttgtctgtta tctacaccga
     1741 tgagggtgag tttgaccgct atctgctgtt gccaaataac ccgaatatgg tcattgtcga
     1801 caccaaaatc gtcgctggcg cacctgcacg tctgttagcg gcgggtatcg gcgatgcgct
     1861 ggcaacctgg tttgaagcgc gtgcctgctc tcgtagcggc gcgaccacca tggcgggcgg
     1921 caagtgcacc caggctgcgc tggcactggc tgaactgtgc tacaacaccc tgctggaaga
     1981 aggcgaaaaa gcgatgcttg ctgccgaaca gcatgtagtg actccggcgc tggagcgcgt
     2041 gattgaagcg aacacctatt tgagcggtgt tggttttgaa agtggtggtc tggctgcggc
     2101 gcacgcagtg cataacggcc tgaccgctat cccggacgcg catcactatt atcacggtga
     2161 aaaagtggca ttcggtacgc tgacgcagct ggttctggaa aatgcgccgg tggaggaaat
     2221 cgaaaccgta gctgccctta gccatgcggt aggtttgcca ataactctcg ctcaactgga
     2281 tattaaagaa gatgtcccgg cgaaaatgcg aattgtggca gaagcggcat gtgcagaagg
     2341 tgaaaccatt cacaacatgc ctggcggcgc gacgccagat caggtttacg ccgctctgct
     2401 ggtagccgac cagtacggtc agcgtttcct gcaagagtgg gaataa
//