LOCUS       BBa_K1172909            3281 bp    DNA     linear       09-Jun-2024
DEFINITION  Biosafety-System araCtive (AraC)
ACCESSION   BBa_K1172909
VERSION     BBa_K1172909.1  
FEATURES             Location/Qualifiers
     promoter        1..122
                     /label=BBa_K914004 Rhamnose promoter
     RBS             129..140
                     /label=RBS BBa_B0034
     CDS             144..1022
                     /label=araC 
     RBS             1029..1040
                     /label=RBS BBa_B0034
     CDS             1044..2123
                     /label=BBa_K1172901 alanine racemase (alr)
     stem_loop       2132..2260
                     /label=BBa_B0015 double Terminator
     RBS             2407..2418
                     /label=RBS BBa_B0034
     promoter        2269..2398
                     /label=arabinose promoter pBAD
     CDS             2425..3143
                     /label=GFP BBa_E0040
     stem_loop       3153..3281
                     /label=double terminator BBa_B0015
ORIGIN
        1 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
       61 attttcctgt cagtaacgag aaggtcgcgt attcaggcgc tttttagact ggtcgtaatg
      121 aatactagaa agaggagaaa caaatggctg aagcgcaaaa tgatcccctg ctgccgggat
      181 actcgtttaa cgcccatctg gtggcgggtt taacgccgat tgaggccaac ggttatctcg
      241 atttttttat cgaccgaccg ctgggaatga aaggttatat tctcaatctc accattcgcg
      301 gtcagggggt ggtgaaaaat cagggacgag aatttgtctg ccgaccgggt gatattttgc
      361 tgttcccgcc aggagagatt catcactacg gtcgtcatcc ggaggctcgc gaatggtatc
      421 accagtgggt ttactttcgt ccgcgcgcct actggcatga atggcttaac tggccgtcaa
      481 tatttgccaa tacgggtttc tttcgcccgg atgaagcgca ccagccgcat ttcagcgacc
      541 tgtttgggca aatcattaac gccgggcaag gggaagggcg ctattcggag ctgctggcga
      601 taaatctgct tgagcaattg ttactgcggc gcatggaagc gattaacgag tcgctccatc
      661 caccgatgga taatcgggta cgcgaggctt gtcagtacat cagcgatcac ctggcagaca
      721 gcaattttga tatcgccagc gtcgcacagc atgtttgctt gtcgccgtcg cgtctgtcac
      781 atcttttccg ccagcagtta gggattagcg tcttaagctg gcgcgaggac caacgcatta
      841 gtcaggcgaa gctgcttttg agcactaccc ggatgcctat cgccaccgtc ggtcgcaatg
      901 ttggttttga cgatcaactc tatttctcgc gagtatttaa aaaatgcacc ggggccagcc
      961 cgagcgagtt tcgtgccggt tgtgaagaaa aagtgaatga tgtagccgtc aagttgtcat
     1021 aatactagaa agaggagaaa caaatgcaag cggcaactgt tgtgattaac cgccgcgctc
     1081 tgcgacacaa cctgcaacgt cttcgtgaac tggcccctgc cagtaaaatg gttgcggtgg
     1141 tgaaagcgaa cgcttatggt cacggtcttc ttgagaccgc gcgaacgctc cccgatgctg
     1201 acgcctttgg cgtagcccgt ctcgaagaag ctctgcgact gcgtgcgggg ggaatcacca
     1261 aacctgtact gttactcgaa ggcttttttg atgccagaga tctgccgacg atttctgcgc
     1321 aacattttca taccgccgtg cataacgaag aacagctggc tgcgctggaa gaggctagcc
     1381 tggacgagcc ggttaccgtc tggatgaaac tcgataccgg tatgcaccgt ctgggcgtaa
     1441 ggccggaaca ggctgaggcg ttttatcatc gcctgaccca gtgcaaaaac gttcgtcagc
     1501 cggtgaatat cgtcagccat tttgcgcgcg cggatgaacc aaaatgtggc gcaaccgaga
     1561 aacaactcgc tatctttaat accttttgcg aaggcaaacc tggtcaacgt tccattgccg
     1621 cgtcgggtgg cattctgctg tggccacagt cgcattttga ctgggtgcgc ccgggcatca
     1681 ttctttatgg cgtctcgccg ctggaagatc gctccaccgg tgccgatttt ggctgtcagc
     1741 cagtgatgtc actaacctcc agcctgattg ccgtgcgtga gcataaagcc ggagagcctg
     1801 ttggttatgg tggaacctgg gtaagcgaac gtgatacccg tcttggcgta gtcgcgatgg
     1861 gctatggcga tggttatccg cgcgccgcgc cgtccggtac gccagtgctg gtgaacggtc
     1921 gcgaagtacc gattgtcggg cgcgtggcga tggatatgat ctgcgtagac ttaggtccac
     1981 aggcgcagga caaagccggg gatccggtca ttttatgggg cgaaggtttg cccgtagaac
     2041 gtatcgctga aatgacgaaa gtaagcgctt acgaacttat tacgcgcctg acttcaaggg
     2101 tcgcgatgaa atacgtggat taatactaga gccaggcatc aaataaaacg aaaggctcag
     2161 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     2221 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagac attgattatt
     2281 tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta gcggatccta
     2341 cctgacgctt tttatcgcaa ctctctactg tttctccata ccgttttttt gggctagcta
     2401 ctagagaaag aggagaaata ctagatgcgt aaaggagaag aacttttcac tggagttgtc
     2461 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
     2521 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
     2581 ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg
     2641 agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat
     2701 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc
     2761 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa
     2821 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc
     2881 atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa
     2941 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
     3001 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac
     3061 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc
     3121 atggatgaac tatacaaata ataatactag agccaggcat caaataaaac gaaaggctca
     3181 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     3241 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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