LOCUS BBa_K1175028 4668 bp DNA linear 18-May-2024 DEFINITION Strong pBAD promoter BBa_K206000 attached to SecI pump BBa_K215104 ACCESSION BBa_K1175028 VERSION BBa_K1175028.1 FEATURES Location/Qualifiers promoter 1..131 /label=promoter misc_feature 40..57 /label=AraI1 misc_feature 61..78 /label=AraI2 promoter 1..130 /label=BBa_K206000 RBS 141..156 /label=native RBS CDS 157..1887 /label=prtD CDS 139..1887 /label=BBa_K215100 RBS 1898..1910 /label=native rbs CDS 1911..3257 /label=prtE CDS 1896..3257 /label=BBa_K215101 RBS 3268..3279 /label=Native RBS CDS 3280..4668 /label=prtF CDS 3266..4668 /label=BBa_K215102 misc_feature 139..4668 /label=BBa_K215104 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagag gggagagagc cctgccatga acgcgtctag cgaacgtgac 181 cgttctctgt ttggtgtgct gcgtcagttc cgccgctcct tttggtctgt tggcatcttc 241 agcgcggtca tcaatgttct gatgctggca cctagcgtct acatgctcca ggtttacgac 301 cgtgtcctcg ccagcggcaa cggtattact ctgctgatgc tcacgctcct gatggcgggt 361 ctgtgcgcct tcatgggtgc tctcgaatgg gttcgcagcc tgctggtagt tcgtctcggt 421 actcgcatcg acctcgcgct caatcaagac gtttttaacg cggctttcgc gcgtaatctg 481 gaagctggtg atggccgtgc cggtctggcg ctgaccgacc tcaccctgct ccgtcaattc 541 atcacgggta atgcactgtt cgcgttcttc gacgttccgt ggttcccgct ctttctcctc 601 gtactgttcc tgctgcaccc ttggctcggc atgctcgcac tgggtggtac ggttgtacct 661 ggtggcgtag gcctcgcaga accggcgtct gaccagtcta ccgcgggtgg ctctaatcaa 721 caaagccaac aagccactca tctcgcggat gcgcagctcc gtaatgcgga cgttatcgaa 781 gcgatgggta tgctcggtaa cctccgtcgt cgctggctgg cgcgtcacta ccgctttatc 841 tccctccaaa atctcgcttc cgagcgcgca gccgccgttg gtggtgcgtc caaatactct 901 cgtattgcac tccaaagcct catgctgggc ctcggtgcgc tcctcgcgat cgacggcaaa 961 atcacgccgg gtatgatgat tgcgggctct atcctcgtcg gtcgtgttct gtccccgatt 1021 gaccagctga tcggtgtttg gaaacagtgg tcttctgccc gcattgcgtg gcagcgcctg 1081 acccgtctga ttgccgcata tccgccacgc cctgcggcta tggctctccc agcgccggaa 1141 ggccacctgt ccgttgagca agtttctctg cgtaccgcac aaggcaatac tcgcctgcaa 1201 aacatccatt tctccctgca agcgggtgag accctggtta tcctgggcgc ctctggttct 1261 ggcaaatctt ctctcgctcg tctgctcgtg ggtgcacaat cccctactca aggtaaagtt 1321 cgcctcgatg gtgcggacct gaaccaagtt gacaaaaaca ccttcggtcc gaccattggt 1381 tacctcccgc aggacgttca actgttcaaa ggtagcctgg cggaaaacat tgcccgtttc 1441 ggcgacgccg acccggaaaa ggttgttgcg gcagctaagc tcgccggtgt tcacgaactc 1501 atcctgtctc tgcctaatgg ttacgacact gagctgggtg acggcggtgg cggcctgtct 1561 ggtggtcaac gtcagcgtat cggtctcgca cgcgcaatgt acggcgaccc gtgtctcctc 1621 attctggacg aaccgaacgc atctctggac tctgagggtg accaagcact catgcaggct 1681 attgtggcgc tccagaaacg tggtgcaacc gttgtactca ttacgcaccg tccggcactc 1741 accaccctcg ctcagaaaat cctgatcctc cacgagggtc aacagcaacg tatgggcctg 1801 gcccgtgacg ttctgacgga actccagcag cgctccgcgg caaaccaggc gcgtatgaac 1861 ccgaccgcag caatgccaca gtaataatac tagagagcag ggatctcgtg atgactggta 1921 tggatattac cactcaggac gaactcaacg aagctgccat gcgtgaccgt gcgagccgtg 1981 acgaagaacg tgcgctccgt ctgggttggt ggctcgtcct cgcgggtttc ggtggtttcc 2041 tcctgtgggc gctcctggct ccactggaca agggtgttgc ggttcagggt aacgttgtag 2101 tttccggtaa ccgtaaagtt atccagcaca tgcaaggcgg tattgttgac cgcatccaag 2161 ttaaggacgg cgaccgtgtt gccgctggtc aggttctgct cactctcaat gcagtcgacg 2221 ctcgtaccac ctccgagggc ctgggttctc agtacgatca gctcatcgcg cgtgaagcgc 2281 gcctcctcgc cgagcagcgt aaccagtctt ctctcgctgc aacgccacgc ctgacgcagg 2341 cacgccagcg tccggaaatg gcggcgatta tcgccctcca ggaagacctg ctgcgctctc 2401 gtcagcagtc tctcaaactg gaaattgacg gtgtccgtgc atctatcgat ggtctggaaa 2461 cttctctggg tgctctgcaa aaagtgatgt cttctaaaca gtctgaacaa gcgactctgt 2521 ctcaacagct gcaaggtctg cgtccgctgg ccgcagacaa ctacgttccg cgtaacaaga 2581 tgctcgaaac cgaacgtctc ttcgcccagg tttctggcga gctggcccag acctctggtg 2641 aagttggtcg tacccgtcgt gacattcaac aacagaaact ccgcattgcg cagcgccagc 2701 aggagtacga caaagaagtc aactctgaac tgtctgacgt tcaggccaaa ctgaacgaag 2761 taatctctca gcgtgaaaag gcggacttca atctcgctaa cgtgcaggtt cgtgcgccgg 2821 tggcgggcac tgtggtggat atgaaaatct tcaccgaagg tggcgtaatt gctccgggtc 2881 aagttatgat ggacattgtt ccggaggatc agccactgct ggtagatggc cgtatcccgg 2941 ttgagatggt tgacaaagta tggagcggtc tgccggtcga actgcaattt actgcgtttt 3001 cccaatctac tacgccgcgt gtccctggta ctgtaaccct gctgagcgcg gatcgcctgg 3061 tggacgaaaa agatggcacc ccttactatg gcctccgtat tcaggtgtct gaggaaggta 3121 aacgtagcct ccacggcctg gagattaaac cgggtatgcc tgtccaaggt tttgtgcgta 3181 cgggcgaacg ctctttcatc aactacctct tcaaaccgct catggaccgt atgcacctcg 3241 cactgaccga agaataatac tagagagcgg aaggaagcaa tgcgccgtaa agcagttctg 3301 ctcactgtgg ttctgagcct gtctggtggt agcgcccaag cgatgggtct gctggacgcg 3361 tgggaactcg cgctccgcaa tgatgcgcaa ctgcgtgcgg caggtttcga acgtgacgcg 3421 ggtcaagagg aagttgcgat tggtcgcgca ggcctcctgc cgagcctgca atacacgtac 3481 ggtgcgaact actcccactc caaagttacc cagcgcgatc gtaccctgaa caacaccacg 3541 aaacgtgatt atgataacta cgtttctacg ctgaccctgc gtcagccact cctggactac 3601 gcggcatggg cgcgttatca gcaaggcgta actcgtaaac tgatggcgga ccagcgtttt 3661 cgcgaccgtt cccaggatct gatggttcgt ctgtaccagt cctggagcga agccctgctg 3721 gcgcaggaaa agctgatgct cctcgacgct cagcgtcgtg cataccagga acagctcgca 3781 ctgaatcgcc gtctcctggc tgccggtgag ggcacgcaaa ccgacctgcg tgagaccgaa 3841 gcacgttata ccgtcaccga ggcgcagcgt atcgaacagg aggacaccct cgatgccgcc 3901 atgactgacc tggaaaacat gatgggctct cctctccaga tccaagacct gtctccactg 3961 gctctcgaca ctctcccgga taacgtaacg gaaaaccgta gcctctctca gtggcgtgaa 4021 ctcacggtac gtcacaacgc caaactggca gtacaacgtg agaacgtgga ttactctcgc 4081 tacgaaatcg agcgcaaccg tgcgggccac ctcccgaccc tggacctggt tgcctctacc 4141 cgtaattccc tctccgagag cgaatacaac tacaatcaaa aatacgacac ccagaccgta 4201 ggtctgcaag ttcgtgttcc tctctactct ggcggtgcgg tttctgcgtc tatgcgccaa 4261 gcggcagctg agtatcaaca gtcccaggcg gaactggaca atcagactcg tcagaccttc 4321 gcggagctgc gtcgccagtt caacctgtgc gcaaacggtg ctgcgaaaat ccgtgcttgg 4381 cagatgtctg tagctgctgc cgaagaggcc attcgtgcga cccgtcagtc tgttgcgggt 4441 ggtgaacgta ttaacctgga tgtgctcatg gctgagcagg aatggtataa tgcccgtcgt 4501 gagctgacgg aggttaagta ccgttggctg caagcgtggc tcaatctgcg ctacacggct 4561 ggtactctca acgaacaaga tatgatgcag ctggcagcct ggttccagtc tgcgccggtt 4621 atcaataaaa ccggcatcaa tgctgccacg ggcaacaaga ccaattaa //