BBa_K118003

BBa_K118003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K118003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew Hall
Date created: 2008-07-23 11:00:00
Date modified: 2015-05-08 01:09:36

crtI coding sequence encoding phytoene dehydrogenase



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K118003_sequence (Version 1)

Description

This is the coding sequence of crtI from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts Phytoene to Lycopene.

Notes

The native crtI sequence contains two internal PstI sites. Primers crtImutf1 and crtImutf2 were ordered so that the MABEL protocol (invented for this purpose) could be used to remove these sites. The mutagenic primers were as follows:

* crtImutf1: gtt tac agt aag gtt gcc
* crtImutf2: agt aac tct ctg ttt gtg c

In both cases, the PstI site CTGCAG was converted to CTGCAA, a silent mutation.

Source

Pantoea ananatis genomic DNA

Sequence Annotation Location Component / Role(s)
PstI site removed
PstI site removed
crtI coding sequence
478,483
889,894
1,1479
feature/s_mutation point_mutation
point_mutation feature/s_mutation
CDS feature/cds
igem#experience
Works
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K118003/1