LOCUS BBa_K118025 4962 bp DNA linear 07-May-2024 DEFINITION limonene generator with lac promoter ACCESSION BBa_K118025 VERSION BBa_K118025.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=SacI misc_feature 248..285 /label=CAP binding site misc_feature 332..352 /label=LacI binding site RBS 359..362 /label=rbs CDS 370..600 /label=lacZ' promoter 320..325 /label=-10 promoter 297..302 /label=-35 misc_feature 1..600 /label=BBa_J33207 misc_feature 609..611 /label=SacI RBS 612..618 /label=rbs RBS 609..618 /label=BBa_J15001 CDS 625..2487 /label=dxs coding sequence CDS 625..2490 /label=BBa_K118000 misc_feature 2499..2501 /label=SacI RBS 2502..2508 /label=rbs RBS 2499..2508 /label=BBa_J15001 CDS 2515..4185 /label=(+) limonene synthase coding region misc_feature 2515..2517 /label=start misc_feature 4180..4185 /label=stop CDS 2515..4185 /label=BBa_I742110 misc_feature 4194..4196 /label=SacI RBS 4197..4203 /label=rbs RBS 4194..4203 /label=BBa_J15001 CDS 4210..4959 /label=appY coding sequence CDS 4210..4962 /label=BBa_K118001 ORIGIN 1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac 61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt 121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc 181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag 361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg 421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga 601 tactagagct caaggaggta ctagatgagt tttgatattg ccaaataccc gaccctggca 661 ctggtcgact ccacccagga gttacgactg ttgccgaaag agagtttacc gaaactctgc 721 gacgaactgc gccgctattt actcgacagc gtgagccgtt ccagcgggca cttcgcctcc 781 gggctgggca cggtcgaact gaccgtggcg ctgcactatg tctacaacac cccgtttgac 841 caattgattt gggatgtggg gcatcaggct tatccgcata aaattttgac cggacgccgc 901 gacaaaatcg gcaccatccg tcagaaaggc ggtctgcacc cgttcccgtg gcgcggcgaa 961 agcgaatatg acgtattaag cgtcgggcat tcatcaacct ccatcagtgc cggaattggt 1021 attgcggttg ctgccgaaaa agaaggcaaa aatcgccgca ccgtctgtgt cattggcgat 1081 ggcgcgatta ccgcaggcat ggcgtttgaa gcgatgaatc acgcgggcga tatccgtcct 1141 gatatgctgg tgattctcaa cgacaatgaa atgtcgattt ccgaaaatgt cggcgcgctc 1201 aacaaccatc tggcacagct gctttccggt aagctttact cttcactgcg cgaaggcggg 1261 aaaaaagttt tctctggcgt gccgccaatt aaagagctgc tcaaacgcac cgaagaacat 1321 attaaaggca tggtagtgcc tggcacgttg tttgaagagc tgggctttaa ctacatcggc 1381 ccggtggacg gtcacgatgt gctggggctt atcaccacgc taaagaacat gcgcgacctg 1441 aaaggcccgc agttcctgca tatcatgacc aaaaaaggtc gtggttatga accggcagaa 1501 aaagacccga tcactttcca cgccgtgcct aaatttgatc cctccagcgg ttgtttgccg 1561 aaaagtagcg gcggtttgcc gagctattca aaaatctttg gcgactggtt gtgcgaaacg 1621 gcagcgaaag acaacaagct gatggcgatt actccggcga tgcgtgaagg ttccggcatg 1681 gtcgagtttt cacgtaaatt cccggatcgc tacttcgacg tggcaattgc cgagcaacac 1741 gcggtgacct ttgctgcggg tctggcgatt ggtgggtaca aacccattgt cgcgatttac 1801 tccactttcc tgcaacgcgc ctatgatcag gtgctgcatg acgtggcgat tcaaaagctt 1861 ccggtcctgt tcgccatcga ccgcgcgggc attgttggtg ctgacggtca aacccatcag 1921 ggtgcttttg atctctctta cctgcgctgc ataccggaaa tggtcattat gaccccgagc 1981 gatgaaaacg aatgtcgcca gatgctctat accggctatc actataacga tggcccgtca 2041 gcggtgcgct acccgcgtgg caacgcggtc ggcgtggaac tgacgccgct ggaaaaacta 2101 ccaattggca aaggcattgt gaagcgtcgt ggcgagaaac tggcgatcct taactttggt 2161 acgctgatgc cagaagcggc gaaagtcgcc gaatcgctga acgccacgct ggtcgatatg 2221 cgttttgtga aaccgcttga tgaagcgtta attctggaaa tggccgccag ccatgaagcg 2281 ctggtcaccg tagaagaaaa cgccattatg ggcggcgcag gcagcggcgt gaacgaagtg 2341 ctgatggccc atcgtaaacc agtacccgtg ctgaacattg gcctgccgga cttctttatt 2401 ccgcaaggaa ctcaggaaga aatgcgcgcc gaactcggcc tcgatgccgc tggtatggaa 2461 gccaaaatca aggcctggct ggcataataa tactagagct caaggaggta ctagatgcgt 2521 cgttcagcaa actaccaacc ttcaatttgg gaccacgatt ttttgcagtc attgaatagc 2581 aactatacgg atgaagcata caaaagacga gcagaagagc tgaggggaaa agtgaagata 2641 gcgattaagg atgtaatcga gcctctggat cagttggagc tgattgataa cttgcaaaga 2701 cttggattgg ctcatcgttt tgagactgag attaggaaca tattgaataa tatctacaac 2761 aataataaag attataattg gagaaaagaa aatctgtatg caacctccct tgagtttaga 2821 ctacttagac aacatggcta tcctgtttct caagaggttt tcaatggttt taaagacgac 2881 cagggaggct tcatttgtga tgatttcaag ggaatactga gcttgcatga agcttcgtat 2941 tacagcttag aaggagaaag catcatggag gaggcctggc aatttaccag caaacatctt 3001 aaagaagtga tgatcagcaa gaacatggaa gaggatgtat ttgtagcaga acaagcgaag 3061 cgtgcactgg aactgcctct gcattggaaa gtgccaatgt tagaggcaag gtggttcata 3121 cacatttatg agagaagaga ggacaagaac caccttttac ttgaactggc taagatggag 3181 tttaacactt tgcaggcaat ttaccaggaa gaactaaaag aaatttcagg gtggtggaag 3241 gatacaggtc ttggagagaa attgagcttt gcgaggaaca ggttggtagc gtccttctta 3301 tggagcatgg ggatcgcgtt tgagcctcaa ttcgcctatt gtcggagagt gctcacaatc 3361 tcgatagccc taattacagt gattgatgac atttatgatg tctatggaac attggatgaa 3421 cttgagatat tcactgatgc tgttgagagg tgggacatca attatgcttt gaagcacctt 3481 ccgggctata tgaaaatgtg ttttcttgcg ctttacaact ttgttaatga atttgcttat 3541 tacgttctca aacaacagga ttttgatttg cttctgagca taaaaaatgc atggcttggc 3601 ttaatacaag cctacttggt ggaggcgaaa tggtaccata gcaagtacac accgaaactg 3661 gaagaatact tggaaaatgg attggtatca ataacgggcc ctttaattat aacgatttca 3721 tatctttctg gtacaaatcc aatcattaag aaggaactgg aatttctgga aagtaatcca 3781 gatatagttc actggtcatc caagattttc cgtctgcaag atgatttggg aacttcatcg 3841 gacgagatac agagagggga tgttccgaaa tcaatccagt gttacatgca tgaaactggt 3901 gcctcagagg aagttgctcg tcaacacatc aaggatatga tgagacagat gtggaagaag 3961 gtgaatgcat acacagccga taaagactct cccttgactg gaacaactac tgagttcctc 4021 ttgaatcttg tgagaatgtc ccattttatg tatctacatg gagatgggca tggtgttcaa 4081 aaccaagaga ctatcgatgt cggttttaca ttgctttttc agcccattcc cttggaggac 4141 aaacacatgg ctttcacagc atctcctggc accaaaggct aataatacta gagctcaagg 4201 aggtactaga tggattatgt ttgctccgta gttttcatct gtcaatcatt tgatttaatt 4261 ataaacagga gagttatctc gttcaaaaaa aattcattgt ttattgtaag cgacaaaatt 4321 agaagggagt taccagtatg cccctctaaa ctaagaattg ttgatataga taagaaaaca 4381 tgtttatcct tttttatcga cgtgaataat gagctgcctg gcaaatttac tcttgataag 4441 aatggctata ttgctgaaga ggaacctcca ttatcgcttg ttttttctct gtttgaaggg 4501 attaaaatag cagactcaca ctccctttgg ttaaaagaaa gactatgtat atccttactt 4561 gccatgttca aaaaacgcga aagtgtaaat tcatttatac taacaaatat aaatacattt 4621 acctgtaaaa ttactggaat aatcagtttt aatattgagc ggcaatggca tttaaaagat 4681 attgcggaat tgatttatac gagtgaaagt ttaataaaaa aaagattaag ggatgaagga 4741 acgtcattta ctgaaatatt gagagatact aggatgaggt atgcaaaaaa actcataact 4801 tcaaactctt attctatcaa tgtcgtagcc cagaaatgtg gctataacag tacttcatat 4861 ttcatatgtg catttaaaga ttattatggt gtcacgccat ctcattattt tgagaaaata 4921 atcggcgtca cagatggaat aaacaaaaca attgactaat aa //