BBa_K1184000

BBa_K1184000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1184000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eric Pederson
Date created: 2013-09-03 11:00:00
Date modified: 2016-01-25 02:40:37

KillerRed



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1184000_sequence (Version 1)

Description

KillerRed is a red fluorescent protein that produces reactive oxygen species (ROS) in the presence of yellow-orange light (540-585 nm). KillerRed is engineered from Aequorea victoria GFP (avGFP) to be phototoxic. It has been shown that KillerRed produces superoxide radical anions by reacting with water. Superoxide reacts with the chromophore of KillerRed, causing it to become dark, which ultimately gives rise to a bleaching effect. KillerRed is spectrally similar to mRFP1 with a similar brightness. KillerRed is oligomeric and may form large aggregates.

This sequence is codon optimized for mammalian cells and has 14 rare proline codons for E. coli (CCC) and one rare arginine codon (AGA). Codon optimization should be taken into consideration if large amounts of the protein are required. Expression on a high-copy plasmid has produced detectable fluorescent signal, however.

Notes

This sequence is not codon optimized for E. coli.

Source

Engineered from avGFP. Provided by the Bruchez lab at Carnegie Mellon

Sequence Annotation Location Component / Role(s)
Double Stop
Start
cds
Phototoxic Mutation Glu(70)
Phototoxic Mutation Ser(121)
Chromophore
Phototoxic Mutation Asn(147)
Phototoxic Mutation Ala(163)
718,723
1,3
1,717
208,210
367,369
199,207
439,441
487,489
feature/stop stop_codon
start_codon feature/start
feature/cds CDS
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
feature/misc sequence_feature
igem#experience
Works
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1184000/1