LOCUS       BBa_K1189030            2812 bp    DNA     linear       18-May-2024
DEFINITION  TALE-B with a his6 tag linked to a K coil under an inducible lacI 
            promoter
ACCESSION   BBa_K1189030
VERSION     BBa_K1189030.1  
FEATURES             Location/Qualifiers
     promoter        1..220
                     /label=LacI Promoter (R0010)
     misc_feature    221..226
                     /label=scar site 
     misc_feature    230..247
                     /label=His tag
     CDS             227..2692
                     /label=TALB
     misc_feature    2669..2746
                     /label=Linker
     CDS             2744..2804
                     /label=Kcoil
     misc_feature    2693..2698
                     /label=KasI
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc accaccacca
      241 ccaccacgac tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga
      301 tgacgatgac aagatggccc ccaagaagaa gaggaaggtg ggcattcacc gcggggtacc
      361 tatggtggac ttgaggacac tcggttattc gcaacagcaa caggagaaaa tcaagcctaa
      421 ggtcaggagc accgtcgcgc aacaccacga ggcgcttgtg gggcatggct tcactcatgc
      481 gcatattgtc gcgctttcac agcaccctgc ggcgcttggg acggtggctg tcaaatacca
      541 agatatgatt gcggccctgc ccgaagccac gcacgaggca attgtagggg tcggtaaaca
      601 gtggtcggga gcgcgagcac ttgaggcgct gctgactgtg gcgggtgagc ttagggggcc
      661 tccgctccag ctcgacaccg ggcagctgct gaagatcgcg aagagagggg gagtaacagc
      721 ggtagaggca gtgcacgcct ggcgcaatgc gctcaccggg gcccccttga acctgacccc
      781 agaccaggta gtcgcaatcg cgtcacatga cgggggaaag caagccctgg aaaccgtgca
      841 aaggttgttg ccggtccttt gtcaagacca cggccttaca ccggagcaag tcgtggccat
      901 tgcatcccac gacggtggca aacaggctct tgagacggtt cagagacttc tcccagttct
      961 ctgtcaagcc cacgggctga ctcccgatca agttgtagcg attgcgtcca acggtggagg
     1021 gaaacaagca ttggagactg tccaacggct ccttcccgtg ttgtgtcaag cccacggttt
     1081 gacgcctgca caagtggtcg ccatcgccag ccatgatggc ggtaagcagg cgctggaaac
     1141 agtacagcgc ctgctgcctg tactgtgcca ggatcatgga ctgaccccag accaggtagt
     1201 cgcaatcgcg tcacatgacg ggggaaagca agccctggaa accgtgcaaa ggttgttgcc
     1261 ggtcctttgt caagaccacg gccttacacc ggagcaagtc gtggccattg caaataataa
     1321 cggtggcaaa caggctcttg agacggttca gagacttctc ccagttctct gtcaagccca
     1381 cgggctgact cccgatcaag ttgtagcgat tgcgtccaac ggtggaggga aacaagcatt
     1441 ggagactgtc caacggctcc ttcccgtgtt gtgtcaagcc cacggtttga cgcctgcaca
     1501 agtggtcgcc atcgcctcga atggcggcgg taagcaggcg ctggaaacag tacagcgcct
     1561 gctgcctgta ctgtgccagg atcatggact gaccccagac caggtagtcg caatcgcgtc
     1621 aaacggaggg ggaaagcaag ccctggaaac cgtgcaaagg ttgttgccgg tcctttgtca
     1681 agaccacggc cttacaccgg agcaagtcgt ggccattgca tcccacgacg gtggcaaaca
     1741 ggctcttgag acggttcaga gacttctccc agttctctgt caagcccacg ggctgactcc
     1801 cgatcaagtt gtagcgattg cgtcgcatga cggagggaaa caagcattgg agactgtcca
     1861 acggctcctt cccgtgttgt gtcaagccca cggtttgacg cctgcacaag tggtcgccat
     1921 cgcctccaat attggcggta agcaggcgct ggaaacagta cagcgcctgc tgcctgtact
     1981 gtgccaggat catggactga ccccagacca ggtagtcgca atcgcgtcac atgacggggg
     2041 aaagcaagcc ctggaaaccg tgcaaaggtt gttgccggtc ctttgtcaag accacggcct
     2101 tacaccggag caagtcgtgg ccattgcaag caacatcggt ggcaaacagg ctcttgagac
     2161 ggttcagaga cttctcccag ttctctgtca agcccacggg ctgactcccg atcaagttgt
     2221 agcgattgcg tccaacggtg gagggaaaca agcattggag actgtccaac ggctccttcc
     2281 cgtgttgtgt caagcccacg gtttgacgcc tgcacaagtg gtcgccatcg ccagccatga
     2341 tggcggtaag caggcgctgg aaacagtaca gcgcctgctg cctgtactgt gccaggatca
     2401 tggactgaca cccgaacagg tggtcgccat tgcttctaat gggggaggac ggccagcctt
     2461 ggagtccatc gtagcccaat tgtccaggcc cgatcccgcg ttggctgcgt taacgaatga
     2521 ccatctggtg gcgttggcat gtcttggtgg acgacccgcg ctcgatgcag tcaaaaaggg
     2581 tctgcctcat gctcccgcat tgatcaaaag aaccaaccgg cggattcccg agagaacttc
     2641 ccatcgagtc gcgggatccg atccaaaaaa gaagagaaag gtataatcta ggggcgccgg
     2701 aggtggtggc agtggaggtg gaggttctgg aggtggtggc tctaaaatcg cagctctgaa
     2761 agagaaaatc gcggctctga aagaaaaaat tgccgcgctg aaggaataat aa
//