BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K1196022
1
BBa_K1196022
NADH:ubiquinone reductase (H+-translocating) Device
2013-09-15T11:00:00Z
2015-05-08T01:09:39Z
anammox
This part synthesize ubiquinone reductase.
false
false
_1510_
0
18361
9
Not in stock
false
This part synthesize ubiquinone reductase.
false
Sheng Ding
component2346898
1
BBa_J23100
component2346900
1
BBa_B0030
component2346905
1
BBa_B0012
component2346903
1
BBa_B0010
component2346902
1
BBa_K1196013
annotation2346898
1
BBa_J23100
range2346898
1
1
35
annotation2346902
1
BBa_K1196013
range2346902
1
67
903
annotation2346903
1
BBa_B0010
range2346903
1
912
991
annotation2346900
1
BBa_B0030
range2346900
1
44
58
annotation2346905
1
BBa_B0012
range2346905
1
1000
1040
BBa_K1196013
1
BBa_K1196013
NADH:ubiquinone reductase (H+-translocating)
2013-09-09T11:00:00Z
2015-05-08T01:09:38Z
Hatefi, Y., Ragan, C.I. and Galante, Y.M. The enzymes and the enzyme complexes of the mitochondrial oxidative phosphorylation system. In: Martonosi, A. (Ed.), The Enzymes of Biological Membranes, 2nd ed., vol. 4, Plenum Press, New York, 1985, p. 1-70.
Oxidoreductases;
Acting on NADH or NADPH;
With a quinone or similar compound as acceptor.
false
false
_1510_
0
18792
9
Not in stock
false
Oxidoreductases;
Acting on NADH or NADPH;
With a quinone or similar compound as acceptor.
false
Tao Sun
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_K1196022_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagagctttcatcctttttccattacttacctcaatacatcatgtcaaatatacatatatatatactcatatgtaattcaaattcaaacaatcaataaccgaaataaacctaatacgaacttacttaaacaaaacagcaacaggcactacactgaggactactcgacttttttttttcgcggttattgaccggttgatccattttttcatctatgtaatagtttaactcaattaaccaattcaaataccttaaaatatctaaattcatgtatttgaccttttaacaacattttacattattaccctaaacttttaacttttattcaatttaatctctaaatctaaaacttacacatttattacaatcgagcccaatatatgctagccaaattctccttaacactcctatagctctcaattaatgatattttcacaagaatttcaccttaatttttttattttatcattttagtccctaaactttaaactaactaaatttactttacaaaataatcctatttaacaacaaacttataaacctatcatttaacttcaaaaatatcaaaaatcatcaatggcaaatttcaaaacctttgacaattttacaaaatagtccctgagttagctagattaagctgcaacaatttaaaaaacgggctttagattcactcacatgcatagatcgattgtttggagaagtttgaagctttcctaatcccctcctatggctttcgagcagtggaaagatgaagatggcaaccatttctctttctttccaccttaattcatttttaattactattattttcattatttatttatttatcattttcaacaaaatttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K1196013_sequence
1
ctttcatcctttttccattacttacctcaatacatcatgtcaaatatacatatatatatactcatatgtaattcaaattcaaacaatcaataaccgaaataaacctaatacgaacttacttaaacaaaacagcaacaggcactacactgaggactactcgacttttttttttcgcggttattgaccggttgatccattttttcatctatgtaatagtttaactcaattaaccaattcaaataccttaaaatatctaaattcatgtatttgaccttttaacaacattttacattattaccctaaacttttaacttttattcaatttaatctctaaatctaaaacttacacatttattacaatcgagcccaatatatgctagccaaattctccttaacactcctatagctctcaattaatgatattttcacaagaatttcaccttaatttttttattttatcattttagtccctaaactttaaactaactaaatttactttacaaaataatcctatttaacaacaaacttataaacctatcatttaacttcaaaaatatcaaaaatcatcaatggcaaatttcaaaacctttgacaattttacaaaatagtccctgagttagctagattaagctgcaacaatttaaaaaacgggctttagattcactcacatgcatagatcgattgtttggagaagtttgaagctttcctaatcccctcctatggctttcgagcagtggaaagatgaagatggcaaccatttctctttctttccaccttaattcatttttaattactattattttcattatttatttatttatcattttcaacaaaatt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z