LOCUS       BBa_K1197007            2006 bp    DNA     linear       01-Jun-2024
DEFINITION  IPTG dependent B.subtilis Kill Switch construct
ACCESSION   BBa_K1197007
VERSION     BBa_K1197007.1  
FEATURES             Location/Qualifiers
     promoter        63..68
                     /label=Sigma A-35
     promoter        86..91
                     /label=Sigma A -10
     promoter        1..97
                     /label=BBa_K143012
     RBS             106..117
                     /label=rbs
     RBS             106..117
                     /label=BBa_K143021
     misc_feature    1..117
                     /label=BBa_K143053
     misc_feature    457..462
                     /label=double TAA added
     CDS             124..462
                     /label=BBa_K302033
     RBS             471..482
                     /label=SpoVG
     RBS             471..482
                     /label=BBa_K606061
     CDS             489..1574
                     /label=BBa_I732100
     stem_loop       1594..1637
                     /label=stem_loop
     misc_feature    1583..1662
                     /label=BBa_B0010
     terminator      1583..1662
                     /label=BBa_B0010
     stem_loop       1678..1697
                     /label=T7 TE
     misc_feature    1704..1704
                     /label=stop
     polyA_site      1698..1711
                     /label=polya
     misc_feature    1671..1711
                     /label=BBa_B0012
     terminator      1671..1711
                     /label=BBa_B0012
     terminator      1583..1711
                     /label=BBa_B0015
     misc_feature    471..1711
                     /label=BBa_K1197004
     misc_feature    1..1711
                     /label=BBa_K1197006
     misc_feature    1720..1740
                     /label=Lac0
     promoter        1756..1760
                     /label=-35
     promoter        1779..1784
                     /label=-10
     misc_feature    1791..1811
                     /label=LacO
     promoter        1720..1811
                     /label=BBa_K606040
     misc_feature    1820..1869
                     /label=BBa_K1197000
     stem_loop       1889..1932
                     /label=stem_loop
     misc_feature    1878..1957
                     /label=BBa_B0010
     terminator      1878..1957
                     /label=BBa_B0010
     stem_loop       1973..1992
                     /label=T7 TE
     misc_feature    1999..1999
                     /label=stop
     polyA_site      1993..2006
                     /label=polya
     misc_feature    1966..2006
                     /label=BBa_B0012
     terminator      1966..2006
                     /label=BBa_B0012
     terminator      1878..2006
                     /label=BBa_B0015
     misc_feature    1720..2006
                     /label=BBa_K1197001
ORIGIN
        1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt
       61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac
      121 tagatggtaa gccgatacgt acccgatatg ggcgatctga tttgggttga ttttgacccg
      181 acaaaaggta gcgagcaagc tggacatcgt ccagctgttg tcctgagtcc tttcatgtac
      241 aacaacaaaa caggtatgtg tctgtgtgtt ccttgtacaa cgcaatcaaa aggatatccg
      301 ttcgaagttg ttttatccgg tcaggaacgt gatggcgtag cgttagctga tcaggtaaaa
      361 agtatcgcct ggcgggcaag aggagcaacg aagaaaggaa cagttgcccc agaggaatta
      421 caactcatta aagccaaaat taacgtactg attgggtaat aatactagag aaaggtggtg
      481 aatactagat gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc
      541 agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag
      601 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg
      661 gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc
      721 aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga
      781 tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac
      841 gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag
      901 ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca
      961 gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg
     1021 gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc
     1081 tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag
     1141 gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg
     1201 ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta
     1261 ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag
     1321 acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc
     1381 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc
     1441 tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct
     1501 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     1561 gcgggcagta ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1621 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1681 tcaccttcgg gtgggccttt ctgcgtttat atactagaga aatgtgagca ctcacaattc
     1741 attttgcaaa agttgttgac tttatctaca aggtgtggca taatgtgtgt aattgtgagc
     1801 ggataacaat ttactagaga aatcagatcg cccatatcgg gtacgtatcg gcttaccatc
     1861 tagtattcat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1921 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1981 ttcgggtggg cctttctgcg tttata
//