LOCUS       BBa_K1197010             966 bp    DNA     linear       17-May-2024
DEFINITION  Pveg (K143012) + RBS (SpoVG) + RFP + DT
ACCESSION   BBa_K1197010
VERSION     BBa_K1197010.1  
FEATURES             Location/Qualifiers
     promoter        63..68
                     /label=Sigma A-35
     promoter        86..91
                     /label=Sigma A -10
     promoter        1..97
                     /label=BBa_K143012
     RBS             106..117
                     /label=rbs
     RBS             106..117
                     /label=BBa_K143021
     CDS             124..798
                     /label=mrfp1
     misc_feature    805..829
                     /label=Help:Barcodes
     CDS             124..804
                     /label=BBa_E1010
     stem_loop       849..892
                     /label=stem_loop
     misc_feature    838..917
                     /label=BBa_B0010
     terminator      838..917
                     /label=BBa_B0010
     stem_loop       933..952
                     /label=T7 TE
     misc_feature    959..959
                     /label=stop
     polyA_site      953..966
                     /label=polya
     misc_feature    926..966
                     /label=BBa_B0012
     terminator      926..966
                     /label=BBa_B0012
     terminator      838..966
                     /label=BBa_B0015
     CDS             124..966
                     /label=BBa_K606012
     misc_feature    106..966
                     /label=BBa_K606013
ORIGIN
        1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt
       61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac
      121 tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa
      181 ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa
      241 ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac
      301 atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc
      361 ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc
      421 gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc
      481 tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa
      541 accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt
      601 gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc
      661 acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg
      721 gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt
      781 cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct actagagcca
      841 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      901 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      961 tttata
//
LOCUS       BBa_I13501               843 bp    DNA     linear       17-May-2024
DEFINITION  Screening plasmid intermediate
ACCESSION   BBa_I13501
VERSION     BBa_I13501.1  
FEATURES             Location/Qualifiers
     CDS             1..675
                     /label=mrfp1
     misc_feature    682..706
                     /label=Help:Barcodes
     CDS             1..681
                     /label=BBa_E1010
     stem_loop       726..769
                     /label=stem_loop
     misc_feature    715..794
                     /label=BBa_B0010
     terminator      715..794
                     /label=BBa_B0010
     stem_loop       810..829
                     /label=T7 TE
     misc_feature    836..836
                     /label=stop
     polyA_site      830..843
                     /label=polya
     misc_feature    803..843
                     /label=BBa_B0012
     terminator      803..843
                     /label=BBa_B0012
ORIGIN
        1 atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt
       61 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt
      121 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc
      181 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg
      241 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa
      301 gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac
      361 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc
      421 atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa
      481 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc
      541 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac
      601 atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt
      661 cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact agagccaggc
      721 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      781 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      841 ata
//