LOCUS       BBa_K1212009            1086 bp    DNA     linear       18-May-2024
DEFINITION  pBAD+Riboswitch2+GFP
ACCESSION   BBa_K1212009
VERSION     BBa_K1212009.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     RBS             176..179
                     /label=RBS/Shine Dalgarno
     stem_loop       131..168
                     /label=Theophylline aptamer
     misc_feature    131..186
                     /label=BBa_K1212000
     misc_feature    903..945
                     /label=LVA tag
     misc_feature    834..834
                     /label=Bsa I fix
     misc_feature    187..945
                     /label=BBa_K1212008
     misc_feature    954..1082
                     /label=B0015
     misc_feature    1083..1086
                     /label=Golden Gate Assembly Site
     terminator      954..1086
                     /label=BBa_K1212018
     misc_feature    187..1086
                     /label=BBa_K1212019
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc ggtgatacca gcatcgtctt gatgcccttg gcagcacccc gctgcaggac
      181 aacaagatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      241 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
      301 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
      361 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg
      421 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
      481 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtggtacc
      541 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
      601 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
      661 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta
      721 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
      781 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agatcacatg
      841 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
      901 aggcctgctg caaacgacga aaactacgct ttagtagctt aataatacta gagccaggca
      961 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1021 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1081 tagctt
//