BBa_K1212018 1 BBa_K1212018 B0015 terminator 2013-09-15T11:00:00Z 2015-05-08T01:09:41Z iGEM distribution kit plate B0015 terminator with overhang used in our Golden Gate Assembly. false false _1526_ 0 18049 9 It's complicated false . false Amy Soon annotation2348075 1 Golden Gate Assembly Site range2348075 1 130 133 annotation2348074 1 B0015 range2348074 1 1 129 BBa_K1212001 1 BBa_K1212001 Theophylline Riboswitch (Clone E) 2013-09-15T11:00:00Z 2015-05-08T01:09:41Z Appl. Environ. Microbiol. December 2010 vol. 76 no. 23 7881-7884. Synthetic riboswitch generated through rational design and in vivo screening. Encodes a RNA secondary structure that in the presence of theophylline undergoes a conformational change that makes available the previously sequestered RBS, thus allowing transcription of the downstream sequence. Achieves a 60-fold activation ratio in 2mM theophylline as reported by Topp et al. in: 'Synthetic Riboswitches That Induce Gene Expression in Diverse Bacterial Species.' Appl. Environ. Microbiol. December 2010 vol. 76 no. 23 7881-7884 false false _1526_ 0 18052 9 Not in stock false None. false Aura Ferreiro annotation2363694 1 Theophylline aptamer range2363694 1 1 38 annotation2363695 1 RBS range2363695 1 44 51 BBa_K1212008 1 BBa_K1212008 Golden Gate compatible GFP 2013-09-15T11:00:00Z 2015-05-08T01:09:41Z DNA from iGEM 2013 distribution K145015 with LVA tag fixed for Golden Gate compatibility through site directed mutagenesis. false false _1526_ 0 18052 9 Not in stock false - false Aura Ferreiro annotation2347651 1 LVA tag range2347651 1 717 759 annotation2347666 1 Bsa I fix range2347666 1 648 648 BBa_K1212019 1 BBa_K1212019 GFP+LVA tag + B0015 2013-09-15T11:00:00Z 2015-05-08T01:09:41Z iGEM distribution kit plate GFP+LVA tag + B0015 with BsaI site removed and Golden Gate Assembly site used in our construction. false false _1526_ 0 18049 9 It's complicated false . false Amy Soon component2348089 1 BBa_K1212018 component2348086 1 BBa_K1212008 annotation2348086 1 BBa_K1212008 range2348086 1 1 759 annotation2348089 1 BBa_K1212018 range2348089 1 768 900 BBa_K1212010 1 BBa_K1212010 pBAD+Riboswitch1+GFP 2013-09-15T11:00:00Z 2015-05-08T01:09:41Z pBAD and GFP are from the iGEM distribution kit plates and the riboswitch is from the Gallivan Lab's paper Synthetic riboswitches that induce gene expression in diverse bacterial species. by Topp et al. in Appl Environ Microbiol. Testing construct. GFP expression is controlled by arabinose and theophylline via the pBAD promoter and the theophylline riboswitch. false false _1526_ 0 18049 9 It's complicated false The end of the riboswitch sequence must be directly before the start codon of the coding sequence for GFP. There cannot be a scar. false Amy Soon component2348133 1 BBa_K1212001 component2348140 1 BBa_K1212019 component2348132 1 BBa_K206000 annotation2348140 1 BBa_K1212019 range2348140 1 191 1090 annotation2348133 1 BBa_K1212001 range2348133 1 131 190 annotation2348132 1 BBa_K206000 range2348132 1 1 130 BBa_K206000 1 pBAD pBAD strong 2009-10-13T11:00:00Z 2015-05-08T01:11:23Z The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1]. Released HQ 2013 pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter. false false _307_ 0 4172 9 In stock true There were no special design considerations. false Amelia Hardjasa annotation2049254 1 AraI2 range2049254 1 61 78 annotation2049252 1 promoter range2049252 1 1 131 annotation2049253 1 AraI1 range2049253 1 40 57 BBa_K1212001_sequence 1 ggtgataccagcatcgtcttgatgcccttggcagcaccctgctaaggaggtaacaacaag BBa_K1212018_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagctt BBa_K1212008_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtggtacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagatcacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa BBa_K1212019_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtggtacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagatcacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagctt BBa_K1212010_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagcggtgataccagcatcgtcttgatgcccttggcagcaccctgctaaggaggtaacaacaagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtggtacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagatcacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagctt BBa_K206000_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z