LOCUS       BBa_K1212013            1049 bp    DNA     linear       23-May-2024
DEFINITION  pTet + TBS1 + RBS + GFP
ACCESSION   BBa_K1212013
VERSION     BBa_K1212013.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    131..149
                     /label=TALe binding site
     misc_feature    131..149
                     /label=BBa_K1212003
     misc_feature    154..157
                     /label=conserved
     RBS             150..161
                     /label=BBa_B0034
     misc_feature    878..920
                     /label=LVA tag
     misc_feature    809..809
                     /label=Bsa I fix
     misc_feature    162..920
                     /label=BBa_K1212008
     stem_loop       932..975
                     /label=stem_loop
     misc_feature    921..1000
                     /label=BBa_B0010
     terminator      921..1000
                     /label=BBa_B0010
     stem_loop       1016..1035
                     /label=T7 TE
     misc_feature    1042..1042
                     /label=stop
     polyA_site      1036..1049
                     /label=polya
     misc_feature    1009..1049
                     /label=BBa_B0012
     terminator      1009..1049
                     /label=BBa_B0012
     terminator      921..1049
                     /label=BBa_B0015
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc taaacagata aatagacaaa aagaggagaa aatgcgtaaa ggagaagaac
      181 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
      241 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
      301 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg
      361 gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg
      421 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
      481 agacacgtgc tgaagtcaag tttgaaggtg gtacccttgt taatagaatc gagttaaaag
      541 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
      601 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
      661 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
      721 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
      781 tttcgaaaga tcccaacgaa aagagagatc acatggtcct tcttgagttt gtaacagctg
      841 ctgggattac acatggcatg gatgaactat acaaaaggcc tgctgcaaac gacgaaaact
      901 acgctttagt agcttaataa ccaggcatca aataaaacga aaggctcagt cgaaagactg
      961 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1021 accttcgggt gggcctttct gcgtttata
//