Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Orr Schlesinger
Date created: 2013-09-06 11:00:00
Date modified: 2015-05-08 01:09:43
P.A.S.E. 1 cassette
| Types | DnaRegion |
| Roles | Project engineered_region |
| Sequences | BBa_K1223001_sequence (Version 1) |
Description
A DNA cassette that undergo homologous recombination into E.coli genome that contains homologous regions at each end, Kanamicin resistance gene (KanR) and death operon containing Lambda holin and lysozime genes and controlled by a Lambda cI regulated promoterNotes
/Source
part is synthetic.holin and lysozime originates from lambda bacteriophage.
the homology regions were taken from E.coli BL21 genome
| Sequence Annotation | Location | Component / Role(s) |
| upstream homology region KanR CDS cI regulated promoter P.A.S.E 1 death operaton Lambda lyzosime Lambda holin CDS downstream homology region | 19,73 234,1049 1059,1107 1059,2088 1134,1610 1631,1948 2094,2148 | feature/misc sequence_feature feature/cds CDS feature/promoter promoter operator feature/operator CDS feature/cds feature/cds CDS sequence_feature feature/misc |