LOCUS       BBa_K1223001            2148 bp    DNA     linear       02-May-2024
DEFINITION  P.A.S.E. 1 cassette
ACCESSION   BBa_K1223001
VERSION     BBa_K1223001.1  
FEATURES             Location/Qualifiers
     misc_feature    19..73
                     /label=upstream homology region
     CDS             234..1049
                     /label=KanR CDS
     promoter        1059..1107
                     /label=cI regulated promoter
     misc_feature    1059..2088
                     /label=P.A.S.E 1 death operaton
     CDS             1134..1610
                     /label=Lambda lyzosime
     CDS             1631..1948
                     /label=Lambda holin CDS
     misc_feature    2094..2148
                     /label=downstream homology region
ORIGIN
        1 tcgacgggcc cgggatccgc gtggcccggc gatcttatat tgatcgccta aagtcatcgc
       61 tacactacca ctacgggaca cctgactgat ccttcaactc agcaaaagtt cgatttattc
      121 aacaaagcca cgttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
      181 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
      241 atattcaacg ggaaacgtct tgctcccgtc cgcgcttaaa ctccaacatg gacgctgatt
      301 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct
      361 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
      421 atgatgttac agatgagatg gtccgtctca actggctgac ggagtttatg cctctcccga
      481 ccatcaagca ttttatccgt actcctgatg atgcgtggtt actcaccacc gcgattcctg
      541 ggaaaacagc cttccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
      601 cgctggccgt gttcctgcgc cggttacatt cgattcctgt ttgtaattgt ccttttaaca
      661 gcgatcgtgt atttcgtctt gctcaggcgc aatcacgcat gaataacggt ttggttgatg
      721 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
      781 acaagctctt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
      841 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacgg gtcggaatcg
      901 cagaccgtta ccaggacctt gccattcttt ggaactgcct cggtgagttt tctccttcat
      961 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     1021 ttcatttgat gctcgatgag tttttctaat aaacctgcta acaccgtgcg tgttgactat
     1081 tttacctctg gcggtgataa tggttgcata aacctccaca gtagaagatg gtagaaatca
     1141 ataatcaacg taaggcgttc ctcgatatgc tggcgtggtc ggagggaact gataacggac
     1201 gtcagaaaac cagaaatcat ggttatgacg tcattgtagg cggagagcta ttcactgatt
     1261 actccgatca ccctcgcaaa cttgtcacgc taaacccaaa actcaaatca acaggcgccg
     1321 gacgctacca gcttctttcc cgttggtggg atgcctaccg caagcagctt ggcctgaaag
     1381 acttctctcc gaaaagtcag gacgctgtgg cattgcagca gattaaggag cgtggcgctt
     1441 tacctatgat tgatcgtggt gatatccgtc aggcaatcga ccgttgcagc aatatctggg
     1501 cttcactgcc gggcgctggt tatggtcagt tcgagcataa ggctgacagc ctgattgcaa
     1561 aattcaaaga agcgggcgga acggtcagag agattgatgt atgaggatct attgggggta
     1621 agacctgaag atgccagaaa aacatgacct gttggccgcc attctcgcgg caaaggaaca
     1681 aggcatcggg gcaatccttg cgtttgcaat ggcgtacctt cgcggcagat ataatggcgg
     1741 tgcgtttaca aaaacagtaa tcgacgcaac gatgtgcgcc attatcgcct ggttcattcg
     1801 tgaccttctc gacttcgccg gactaagtag caatctcgct tatataacga gcgtgtttat
     1861 cggctacatc ggtactgact cgattggttc gcttatcaaa cgcttcgctg ctaaaaaagc
     1921 cggagtagaa gatggtagaa atcaataagg atctgatctc caggcatcaa ataaaacgaa
     1981 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     2041 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatagg gatcgcacca
     2101 acggcgacac gctgaaggcc ttcgaaatga atatctttca ctacgaac
//