LOCUS       BBa_K1230003            1009 bp    DNA     linear       10-May-2024
DEFINITION  RBS-GFP-Terminator with LL-37 antimicrobial peptide with modified 
            codon usage for E. coli as prefix
ACCESSION   BBa_K1230003
VERSION     BBa_K1230003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=start
     CDS             1..123
                     /label=BBa_K875009
     RBS             132..146
                     /label=RBS-1\Strong
     RBS             139..143
                     /label=RBS
     misc_feature    132..146
                     /label=BBa_B0030
     RBS             132..146
                     /label=BBa_B0030
     CDS             153..872
                     /label=GFP protein
     CDS             153..872
                     /label=BBa_E0040
     stem_loop       892..935
                     /label=stem_loop
     misc_feature    881..960
                     /label=BBa_B0010
     terminator      881..960
                     /label=BBa_B0010
     stem_loop       976..995
                     /label=T7 TE
     misc_feature    1002..1002
                     /label=stop
     polyA_site      996..1009
                     /label=polya
     misc_feature    969..1009
                     /label=BBa_B0012
     terminator      969..1009
                     /label=BBa_B0012
     misc_feature    132..1009
                     /label=BBa_E0840
ORIGIN
        1 atgctgctgg gcgatttttt tcgcaaaagc aaagaaaaaa ttggcaaaga atttaaacgc
       61 attgtgcagc gcattaaaga ttttctgcgc aacctggtgc cgcgcaccga aagctaataa
      121 taatactaga gattaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg
      181 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
      241 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
      301 ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat
      361 gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg
      421 aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg
      481 ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt
      541 ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac tcacacaatg
      601 tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca
      661 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg
      721 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag
      781 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta
      841 cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga
      901 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      961 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//