LOCUS       BBa_K1230012            1114 bp    DNA     linear       13-May-2024
DEFINITION  rbs-GFP-terminator-pLsrA-LL37
ACCESSION   BBa_K1230012
VERSION     BBa_K1230012.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..741
                     /label=GFP protein
     CDS             22..741
                     /label=BBa_E0040
     stem_loop       761..804
                     /label=stem_loop
     misc_feature    750..829
                     /label=BBa_B0010
     terminator      750..829
                     /label=BBa_B0010
     stem_loop       845..864
                     /label=T7 TE
     misc_feature    871..871
                     /label=stop
     polyA_site      865..878
                     /label=polya
     misc_feature    838..878
                     /label=BBa_B0012
     terminator      838..878
                     /label=BBa_B0012
     misc_feature    1..878
                     /label=BBa_E0840
     RBS             998..1009
                     /label=RBS BBa_B0034
     promoter        887..988
                     /label=BBa_K117002
     misc_feature    1..988
                     /label=BBa_K1230008
     CDS             995..1114
                     /label=LL-37
     misc_feature    995..997
                     /label=Start
     misc_feature    1108..1114
                     /label=Stop
     misc_feature    995..1114
                     /label=BBa_K1230001
ORIGIN
        1 attaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
       61 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      121 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      181 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      241 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      301 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      361 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      421 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      481 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      541 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      601 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      661 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
      721 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc
      781 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      841 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagcaca acatcaactg
      901 agtgcatgcc ttatttaatc atgtaactat tcttacgact tcaaacgtca aacagtctta
      961 acacttattt aattaaaaag aggagaaata ctagatgctg ctgggtgatt tcttccggaa
     1021 atctaaagag aagattggca aagagtttaa aagaattgtc cagagaatca aggatttttt
     1081 gcggaatctt gtacccagga cagagtccta ataa
//