LOCUS       BBa_K1230014            1505 bp    DNA     linear       01-Jun-2024
DEFINITION  rbs-GFP-pLacI-rbs-MarA Generator
ACCESSION   BBa_K1230014
VERSION     BBa_K1230014.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..741
                     /label=GFP protein
     CDS             22..741
                     /label=BBa_E0040
     stem_loop       761..804
                     /label=stem_loop
     misc_feature    750..829
                     /label=BBa_B0010
     terminator      750..829
                     /label=BBa_B0010
     stem_loop       845..864
                     /label=T7 TE
     misc_feature    871..871
                     /label=stop
     polyA_site      865..878
                     /label=polya
     misc_feature    838..878
                     /label=BBa_B0012
     terminator      838..878
                     /label=BBa_B0012
     misc_feature    1..878
                     /label=BBa_E0840
     CDS             887..974
                     /label=end of LacI coding region (inactive)
     misc_feature    887..1086
                     /label=BBa_R0010
     misc_feature    975..1012
                     /label=CAP binding site
     promoter        1023..1028
                     /label=-35
     promoter        1047..1052
                     /label=-10
     misc_feature    1052..1086
                     /label=LacI binding site
     misc_feature    1059..1059
                     /label=start
     promoter        887..1086
                     /label=BBa_R0010
     misc_feature    1099..1102
                     /label=conserved
     RBS             1095..1106
                     /label=BBa_B0034
     misc_feature    887..1106
                     /label=BBa_J04500
     CDS             1113..1505
                     /label=MarA
     misc_feature    1500..1505
                     /label=stop
     misc_feature    1113..1115
                     /label=start
     misc_feature    1244..1244
                     /label=G to C mutation
     CDS             1113..1505
                     /label=BBa_K1230000
     misc_feature    887..1505
                     /label=BBa_K1230004
ORIGIN
        1 attaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
       61 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      121 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      181 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      241 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      301 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      361 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      421 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      481 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      541 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      601 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      661 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
      721 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc
      781 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      841 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagcaat acgcaaaccg
      901 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
      961 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     1021 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     1081 cacacatact agagaaagag gagaaatact agatgacgat gtccagacgc aatactgacg
     1141 ctattaccat tcatagcatt ttggactgga tcgaggacaa cctggaatcg ccactgtcac
     1201 tggagaaagt gtcagagcgt tcgggttact ccaaatggca cctccaacgg atgtttaaaa
     1261 aagaaaccgg tcattcatta ggccaataca tccgcagccg taagatgacg gaaatcgcgc
     1321 aaaagctgaa ggaaagtaac gagccgatac tctatctggc agaacgatat ggcttcgagt
     1381 cgcaacaaac tctgacccga accttcaaaa attactttga tgttccgccg cataaatacc
     1441 ggatgaccaa tatgcagggc gaatcgcgct ttttacatcc attaaatcat tacaacagct
     1501 aataa
//