LOCUS BBa_K1253113 4392 bp DNA linear 17-May-2024 DEFINITION TetR half-life tester ACCESSION BBa_K1253113 VERSION BBa_K1253113.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 88..91 /label=conserved RBS 84..95 /label=BBa_B0034 CDS 105..721 /label=tetR misc_feature 722..755 /label=SsrA misc_feature 762..786 /label=Help:Barcodes CDS 102..761 /label=BBa_C0040 stem_loop 806..849 /label=stem_loop misc_feature 795..874 /label=BBa_B0010 terminator 795..874 /label=BBa_B0010 stem_loop 890..909 /label=T7 TE misc_feature 916..916 /label=stop polyA_site 910..923 /label=polya misc_feature 883..923 /label=BBa_B0012 terminator 883..923 /label=BBa_B0012 misc_feature 84..923 /label=BBa_P0440 CDS 930..1604 /label=mrfp1 misc_feature 1611..1635 /label=Help:Barcodes CDS 930..1635 /label=BBa_E1010 stem_loop 1655..1698 /label=stem_loop misc_feature 1644..1723 /label=BBa_B0010 terminator 1644..1723 /label=BBa_B0010 stem_loop 1739..1758 /label=T7 TE misc_feature 1765..1765 /label=stop polyA_site 1759..1772 /label=polya misc_feature 1732..1772 /label=BBa_B0012 terminator 1732..1772 /label=BBa_B0012 terminator 1644..1772 /label=BBa_B0015 misc_feature 1781..1799 /label=TetR 1 misc_feature 1781..1834 /label=BBa_R0040 promoter 1800..1805 /label=-35 misc_feature 1806..1824 /label=TetR 2 promoter 1823..1828 /label=-10 promoter 1781..1834 /label=BBa_R0040 RBS 1843..1857 /label=RBS-1\Strong RBS 1850..1854 /label=RBS misc_feature 1843..1857 /label=BBa_B0030 RBS 1843..1857 /label=BBa_B0030 CDS 1864..2583 /label=GFP protein CDS 1864..2583 /label=BBa_E0040 stem_loop 2603..2646 /label=stem_loop misc_feature 2592..2671 /label=BBa_B0010 terminator 2592..2671 /label=BBa_B0010 stem_loop 2687..2706 /label=T7 TE misc_feature 2713..2713 /label=stop polyA_site 2707..2720 /label=polya misc_feature 2680..2720 /label=BBa_B0012 terminator 2680..2720 /label=BBa_B0012 misc_feature 1843..2720 /label=BBa_E0840 stem_loop 2740..2783 /label=stem_loop misc_feature 2729..2808 /label=BBa_B0010 terminator 2729..2808 /label=BBa_B0010 stem_loop 2824..2843 /label=T7 TE misc_feature 2850..2850 /label=stop polyA_site 2844..2857 /label=polya misc_feature 2817..2857 /label=BBa_B0012 terminator 2817..2857 /label=BBa_B0012 terminator 2729..2857 /label=BBa_B0015 misc_feature 2866..2884 /label=TetR 1 misc_feature 2866..2919 /label=BBa_R0040 promoter 2885..2890 /label=-35 misc_feature 2891..2909 /label=TetR 2 promoter 2908..2913 /label=-10 promoter 2866..2919 /label=BBa_R0040 misc_feature 2932..2935 /label=conserved RBS 2928..2939 /label=BBa_B0034 misc_feature 2952..2955 /label=conserved RBS 2948..2959 /label=BBa_B0034 misc_feature 4055..4093 /label=LVA CDS 2966..4093 /label=lacI-LVA misc_feature 2966..4093 /label=BBa_C0012 misc_feature 4094..4118 /label=Help:Barcodes CDS 2966..4093 /label=BBa_C0012 stem_loop 4138..4181 /label=stem_loop misc_feature 4127..4206 /label=BBa_B0010 terminator 4127..4206 /label=BBa_B0010 stem_loop 4222..4241 /label=T7 TE misc_feature 4248..4248 /label=stop polyA_site 4242..4255 /label=polya misc_feature 4215..4255 /label=BBa_B0012 terminator 4215..4255 /label=BBa_B0012 misc_feature 2948..4255 /label=BBa_P0412 stem_loop 4275..4318 /label=stem_loop misc_feature 4264..4343 /label=BBa_B0010 terminator 4264..4343 /label=BBa_B0010 stem_loop 4359..4378 /label=T7 TE misc_feature 4385..4385 /label=stop polyA_site 4379..4392 /label=polya misc_feature 4352..4392 /label=BBa_B0012 terminator 4352..4392 /label=BBa_B0012 terminator 4264..4392 /label=BBa_B0015 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gagaaagagg agaaatacta gatgtccaga ttagataaaa 121 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa 181 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa 241 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact 301 tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta 361 gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag 421 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac 481 tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg 541 aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc 601 cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct 661 tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt 721 ccgctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag tgctagtgta 781 gatcactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 841 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 901 gggtgggcct ttctgcgttt atatactaga tggcttcctc cgaagacgtt atcaaagagt 961 tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag 1021 gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag 1081 gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag 1141 cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt 1201 tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact 1261 cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt 1321 ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt 1381 acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg 1441 gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg 1501 gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg 1561 ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt 1621 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 1681 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 1741 ctcaccttcg ggtgggcctt tctgcgttta tatactagag tccctatcag tgatagagat 1801 tgacatccct atcagtgata gagatactga gcactactag agattaaaga ggagaaatac 1861 tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat 1921 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac 1981 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca 2041 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa 2101 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt 2161 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt 2221 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac 2281 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat 2341 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca 2401 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat 2461 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc 2521 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa 2581 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 2641 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 2701 tgggcctttc tgcgtttata tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 2761 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 2821 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtccct atcagtgata 2881 gagattgaca tccctatcag tgatagagat actgagcact actagagaaa gaggagaaat 2941 actagagaaa gaggagaaat actagatggt gaatgtgaaa ccagtaacgt tatacgatgt 3001 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca 3061 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc 3121 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc 3181 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca 3241 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc 3301 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga 3361 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga 3421 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact 3481 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt 3541 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca 3601 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac 3661 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat 3721 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc 3781 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat 3841 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca 3901 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac 3961 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 4021 ggcacgacag gtttcccgac tggaaagcgg gcaggctgca aacgacgaaa actacgcttt 4081 agtagcttaa taactctgat agtgctagtg tagatctcta ctagagccag gcatcaaata 4141 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 4201 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 4261 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 4321 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 4381 tctgcgttta ta //