BBa_K1255003 1 BBa_K1255003 pConst + Riboswitch + BSMT1 2014-06-15T11:00:00Z 2015-05-08T01:09:45Z BSTM1 opt. comes from <i>Petunia x hybrida</i> BSMT1 activated with a constitutive promotor, regulated a riboswitch Description: BSMT1 opt. (BBa_K1255001) encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase, which is a molecule catalyzes the salicylic acid to methyl salicylate. In order to do it, there must be added 2mM of salicylic acid to produce a winter green aroma. Besides this device has a constitutive promotor (BBa_J23119), it is regulated by a Riboswitch (BBa_K115017) that is a RBS but with a loop that unfolds at a temperature around of 32?? C. false false _1611_ 0 19659 9 It's complicated false BSTM1 opt. was optimized for its expression in <i>E. coli</i> false Antonio Cardenas, Irina Romero, Dulce Valdez component2379136 1 BBa_K1255001 component2379134 1 BBa_K115017 component2379132 1 BBa_J23119 annotation2379134 1 BBa_K115017 range2379134 1 44 126 annotation2379132 1 BBa_J23119 range2379132 1 1 35 annotation2379136 1 BBa_K1255001 range2379136 1 133 1206 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_K115017 1 BBa_K115017 RNA thermometer (ROSE 32??C) 2008-08-19T11:00:00Z 2015-05-08T01:09:28Z Based on ROSE RNA thermometer sequences from Rfam database. Released HQ 2013 An RNA thermometer that theoretically switches on translation at 32 degrees Celcius and switched of translation below that temperature. Still to be tested. false false _223_ 0 3006 9 In stock true a lot, will be added soon. true Bastiaan van den Berg annotation1972920 1 SD range1972920 1 77 82 BBa_K1255001 1 BBa_K1255001 BSMT1 Opt. 2014-06-12T11:00:00Z 2015-05-08T01:09:45Z http://parts.igem.org/Part:BBa_J45004 BBa_J45004 optimized. According to our tests, there must be added between 10mM and 20mM of salicylic acid to produce a wintergreen aroma. For further information check the experience section in this page. false false _1611_ 0 19636 9 Not in stock false This CDS has being optimized for <i>E. coli</i>, from the piece BBa_J45004 of the MIT group of 2006. false Antonio Cardenas, Irina Romero, Dulce Valdez annotation2379061 1 BSMT1 Opt. range2379061 1 1 1074 BBa_K1255001_sequence 1 atggaagtggttgaagttctgcacatgaacggtggtaatggtgattcgtcgtatgccaataacagcctggtccaacaaaaagtgattctgatgaccaaaccgattacggaacaggcaatgatcgatctgtatagctctctgttcccggaaaccctgtgcattgcggacctgggctgtagcctgggtgccaacacgtttctggtggtttctcaactggtcaaaatcgtggaaaaagaacgtaaaaaacatggcttcaaaagtccggaattttacttccactttaatgatctgccgggcaacgacttcaataccctgtttcagtcactgggtgcgttccaagaagatctgcgtaaacatattggcgaatcttttggtccgtgctttttcagtggcgtgccgggttccttctatacgcgcctgtttccgtcaaaatcgctgcactttgtgtatagttcctactcactgatgtggctgtcgcaggttccgaacggcatcgaaaacaacaagggtaacatctacatggcacgcaccagcccgctgtctgtgatcaaagcttactacaaacaatacgaaatcgatttcagtaactttctgaaataccgttccgaagaactgatgaaaggcggtaaaatggttctgaccctgctgggccgcgaaagcgaagacccgacgtctaaagaatgctgttatatttgggaactgctggcgatggccctgaacaaactggttgaagaaggtctgattaaagaagaaaaagtcgatgcgttcaatatcccgcagtataccccgagtccggccgaagttaaatacatcgtcgaaaaagaaggttcctttaccatcaaccgtctggaaacgtcacgcgtccattggaatgcgtcgaacaatgagaaaaacggcggttataatgtttcacgttgtatgcgcgcagtggctgaaccgctgctggtttcgcatttcgataaagaactgatggacctggtctttcacaaatacgaagaaatcgtgagcgactgtatgtccaaagaaaacacggaatttatcaatgttatcatctccctgaccaaaatcaattaa BBa_K115017_sequence 1 ccgggcgcccttcgggggcccggcggagacgggcgccggaggtgtccgacgcctgctcgtccagtctttgctcagtggaggat BBa_K1255003_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagccgggcgcccttcgggggcccggcggagacgggcgccggaggtgtccgacgcctgctcgtccagtctttgctcagtggaggattactagatggaagtggttgaagttctgcacatgaacggtggtaatggtgattcgtcgtatgccaataacagcctggtccaacaaaaagtgattctgatgaccaaaccgattacggaacaggcaatgatcgatctgtatagctctctgttcccggaaaccctgtgcattgcggacctgggctgtagcctgggtgccaacacgtttctggtggtttctcaactggtcaaaatcgtggaaaaagaacgtaaaaaacatggcttcaaaagtccggaattttacttccactttaatgatctgccgggcaacgacttcaataccctgtttcagtcactgggtgcgttccaagaagatctgcgtaaacatattggcgaatcttttggtccgtgctttttcagtggcgtgccgggttccttctatacgcgcctgtttccgtcaaaatcgctgcactttgtgtatagttcctactcactgatgtggctgtcgcaggttccgaacggcatcgaaaacaacaagggtaacatctacatggcacgcaccagcccgctgtctgtgatcaaagcttactacaaacaatacgaaatcgatttcagtaactttctgaaataccgttccgaagaactgatgaaaggcggtaaaatggttctgaccctgctgggccgcgaaagcgaagacccgacgtctaaagaatgctgttatatttgggaactgctggcgatggccctgaacaaactggttgaagaaggtctgattaaagaagaaaaagtcgatgcgttcaatatcccgcagtataccccgagtccggccgaagttaaatacatcgtcgaaaaagaaggttcctttaccatcaaccgtctggaaacgtcacgcgtccattggaatgcgtcgaacaatgagaaaaacggcggttataatgtttcacgttgtatgcgcgcagtggctgaaccgctgctggtttcgcatttcgataaagaactgatggacctggtctttcacaaatacgaagaaatcgtgagcgactgtatgtccaaagaaaacacggaatttatcaatgttatcatctccctgaccaaaatcaattaa BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z