LOCUS       BBa_K127009             2050 bp    DNA     linear       23-May-2024
DEFINITION  J23105 B0034 C0061 B0015 I732910
ACCESSION   BBa_K127009
VERSION     BBa_K127009.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23105
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    641..672
                     /label=LVA
     CDS             62..640
                     /label=luxI
     misc_feature    62..679
                     /label=BBa_C0061
     misc_feature    680..704
                     /label=Help:Barcodes
     CDS             62..679
                     /label=BBa_C0061
     stem_loop       724..767
                     /label=stem_loop
     misc_feature    713..792
                     /label=BBa_B0010
     terminator      713..792
                     /label=BBa_B0010
     stem_loop       808..827
                     /label=T7 TE
     misc_feature    834..834
                     /label=stop
     polyA_site      828..841
                     /label=polya
     misc_feature    801..841
                     /label=BBa_B0012
     terminator      801..841
                     /label=BBa_B0012
     terminator      713..841
                     /label=BBa_B0015
     stem_loop       861..904
                     /label=stem_loop
     misc_feature    850..929
                     /label=BBa_B0010
     terminator      850..929
                     /label=BBa_B0010
     stem_loop       945..964
                     /label=T7 TE
     misc_feature    971..971
                     /label=stop
     polyA_site      965..978
                     /label=polya
     misc_feature    938..978
                     /label=BBa_B0012
     terminator      938..978
                     /label=BBa_B0012
     terminator      850..978
                     /label=BBa_B0015
     misc_feature    987..1023
                     /label=P(Lac) IQ
     promoter        989..994
                     /label=-35
     promoter        1012..1017
                     /label=-10
     promoter        987..1023
                     /label=BBa_I14032
     misc_feature    1036..1039
                     /label=conserved
     RBS             1032..1043
                     /label=BBa_B0034
     misc_feature    1050..1051
                     /label=prefix
     misc_feature    1223..1223
                     /label=T
     misc_feature    1541..1541
                     /label=A
     CDS             1050..1799
                     /label=luxR
     misc_feature    1050..1805
                     /label=BBa_C0062
     misc_feature    1806..1830
                     /label=Help:Barcodes
     CDS             1050..1805
                     /label=BBa_C0062
     stem_loop       1850..1893
                     /label=stem_loop
     misc_feature    1839..1918
                     /label=BBa_B0010
     terminator      1839..1918
                     /label=BBa_B0010
     stem_loop       1934..1953
                     /label=T7 TE
     misc_feature    1960..1960
                     /label=stop
     polyA_site      1954..1967
                     /label=polya
     misc_feature    1927..1967
                     /label=BBa_B0012
     terminator      1927..1967
                     /label=BBa_B0012
     misc_feature    1032..1967
                     /label=BBa_I0462
     misc_feature    1976..1995
                     /label=LuxR/HSL
     promoter        1995..2000
                     /label=-35
     promoter        2017..2022
                     /label=-10
     misc_feature    2028..2028
                     /label=start
     misc_feature    1976..2030
                     /label=BBa_R0062
     promoter        1976..2030
                     /label=BBa_R0062
     misc_feature    987..2030
                     /label=BBa_J09855
     misc_feature    2043..2046
                     /label=conserved
     RBS             2039..2050
                     /label=BBa_B0034
     misc_feature    850..2050
                     /label=BBa_I732910
ORIGIN
        1 tttacggcta gctcagtcct aggtactatg ctagctacta gagaaagagg agaaatacta
       61 gatgactata atgataaaaa aatcggattt tttggcaatt ccatcggagg agtataaagg
      121 tattctaagt cttcgttatc aagtgtttaa gcaaagactt gagtgggact tagttgtaga
      181 aaataacctt gaatcagatg agtatgataa ctcaaatgca gaatatattt atgcttgtga
      241 tgatactgaa aatgtaagtg gatgctggcg tttattacct acaacaggtg attatatgct
      301 gaaaagtgtt tttcctgaat tgcttggtca acagagtgct cccaaagatc ctaatatagt
      361 cgaattaagt cgttttgctg taggtaaaaa tagctcaaag ataaataact ctgctagtga
      421 aattacaatg aaactatttg aagctatata taaacacgct gttagtcaag gtattacaga
      481 atatgtaaca gtaacatcaa cagcaataga gcgattttta aagcgtatta aagttccttg
      541 tcatcgtatt ggagacaaag aaattcatgt attaggtgat actaaatcgg ttgtattgtc
      601 tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat gctgcaaacg acgaaaacta
      661 cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat
      721 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      781 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      841 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      961 ggcctttctg cgtttatata ctagagtggt gcaaaacctt tcgcggtatg gcatgatagc
     1021 gcctactaga gaaagaggag aaatactaga tgaaaaacat aaatgccgac gacacataca
     1081 gaataattaa taaaattaaa gcttgtagaa gcaataatga tattaatcaa tgcttatctg
     1141 atatgactaa aatggtacat tgtgaatatt atttactcgc gatcatttat cctcattcta
     1201 tggttaaatc tgatatttca atcctagata attaccctaa aaaatggagg caatattatg
     1261 atgacgctaa tttaataaaa tatgatccta tagtagatta ttctaactcc aatcattcac
     1321 caattaattg gaatatattt gaaaacaatg ctgtaaataa aaaatctcca aatgtaatta
     1381 aagaagcgaa aacatcaggt cttatcactg ggtttagttt ccctattcat acggctaaca
     1441 atggcttcgg aatgcttagt tttgcacatt cagaaaaaga caactatata gatagtttat
     1501 ttttacatgc gtgtatgaac ataccattaa ttgttccttc tctagttgat aattatcgaa
     1561 aaataaatat agcaaataat aaatcaaaca acgatttaac caaaagagaa aaagaatgtt
     1621 tagcgtgggc atgcgaagga aaaagctctt gggatatttc aaaaatatta ggttgcagtg
     1681 agcgtactgt cactttccat ttaaccaatg cgcaaatgaa actcaataca acaaaccgct
     1741 gccaaagtat ttctaaagca attttaacag gagcaattga ttgcccatac tttaaaaatt
     1801 aataacactg atagtgctag tgtagatcac tactagagcc aggcatcaaa taaaacgaaa
     1861 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1921 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagacctg
     1981 taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataaa tactagagaa
     2041 agaggagaaa
//