FLAG

BBa_K128000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K128000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sara Mouradian
Date created: 2008-07-09 11:00:00
Date modified: 2015-05-08 01:09:46

FLAG affinity tag



Types
DnaRegion

Roles
tag

Tag

Sequences BBa_K128000_sequence (Version 1)

Description

This is the polypeptide protein FLAG tag. It is used in antibody binding assays if there is no antibody against the studied protein. It should be put at either the N terminus or the C terminus of the protein This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.

Notes

Tags are almost always used in conjunction with another protein (the one being studied), so we used the modified Silver BioBrick prefix and suffix to make the construction of funcitonal fusion peptides possible

Source

This sequence is easily recognized by many antibodies.

Sequence Annotation Location Component / Role(s)
Flag tag
1,25
tag feature/tag
igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K128000/1