LOCUS       BBa_K1300009            3031 bp    DNA     linear       20-May-2024
DEFINITION  araC gene + pBAD + cI repressor
ACCESSION   BBa_K1300009
VERSION     BBa_K1300009.1  
FEATURES             Location/Qualifiers
     CDS             1..879
                     /label=AraC
     misc_feature    909..924
                     /label=AraO
     misc_feature    1066..1077
                     /label=AraO1
     promoter        1153..1181
                     /label=AraPromoter
     promoter        1..1209
                     /label=BBa_K808000
     misc_feature    1222..1225
                     /label=conserved
     RBS             1218..1229
                     /label=BBa_B0034
     CDS             1236..1955
                     /label=GFP protein
     CDS             1236..1955
                     /label=BBa_E0040
     stem_loop       1975..2018
                     /label=stem_loop
     misc_feature    1964..2043
                     /label=BBa_B0010
     terminator      1964..2043
                     /label=BBa_B0010
     stem_loop       2059..2078
                     /label=T7 TE
     misc_feature    2085..2085
                     /label=stop
     polyA_site      2079..2092
                     /label=polya
     misc_feature    2052..2092
                     /label=BBa_B0012
     terminator      2052..2092
                     /label=BBa_B0012
     terminator      1964..2092
                     /label=BBa_B0015
     promoter        2101..2230
                     /label=BBa_I13453
     misc_feature    2243..2246
                     /label=conserved
     RBS             2239..2250
                     /label=BBa_B0034
     CDS             2260..2967
                     /label=cI lambda
     misc_feature    2968..3000
                     /label=LVA
     misc_feature    3007..3031
                     /label=Help:Barcodes
     CDS             2257..3031
                     /label=BBa_C0051
     misc_feature    1218..3031
                     /label=BBa_K611014
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 tgggctaact actagagaaa gaggagaaat actagatgcg taaaggagaa gaacttttca
     1261 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
     1321 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
     1381 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc
     1441 aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc
     1501 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac
     1561 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg
     1621 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca
     1681 atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac
     1741 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
     1801 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
     1861 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
     1921 ttacacatgg catggatgaa ctatacaaat aataatacta gagccaggca tcaaataaaa
     1981 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2041 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag
     2101 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
     2161 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
     2221 ttgggctagc tactagagaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta
     2281 acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat
     2341 gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt
     2401 ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa
     2461 attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg
     2521 tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct
     2581 catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag
     2641 agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt
     2701 aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc
     2761 gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat
     2821 gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac
     2881 ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct
     2941 agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct
     3001 taataacgct gatagtgcta gtgtagatcg c
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