LOCUS       BBa_K131027             2960 bp    DNA     linear       19-May-2024
DEFINITION  ColE3 operon
ACCESSION   BBa_K131027
VERSION     BBa_K131027.1  
FEATURES             Location/Qualifiers
     promoter        203..209
                     /label=-35 region
     promoter        226..231
                     /label=-10 region
     RBS             302..307
                     /label=RBS
     CDS             316..1967
                     /label=ColicinE3
     RBS             1973..1978
                     /label=RBS
     CDS             1981..2238
                     /label=Immunity protein E3
     CDS             2405..2662
                     /label=Immunity protein E8
     CDS             2724..2867
                     /label=Lysis protein
ORIGIN
        1 agacctggca tgagtggaag cgggacgaac agcacaggca acaacaacgc cgccccgggc
       61 acttccgggg catgagtatg tgatatccgg ggctgcaccc cggaccccgc caacacatca
      121 cgggccacaa aattttttgt ggcccgctct gcgttttcta agtgttatcc ctcctgattt
      181 ctaaaaaatt ttccacctga acttgacaga aaaaacgatg acgagtactt tttgatctgt
      241 acataaaccc agtggtttta tgtacagtat taatcgtgta atcaattgtt ttaacgctta
      301 aaagagggaa tttttatgag cggtggcgat ggacgcggcc ataacacggg cgcgcatagc
      361 acaagtggta acattaatgg tggcccgacc gggcttggtg taggtggtgg tgcttctgat
      421 ggctccggat ggagttcgga aaataacccg tggggtggtg gttccggtag cggcattcac
      481 tggggtggtg gttccggtca tggtaatggc ggggggaatg gtaattccgg tggtggttcg
      541 ggaacaggcg gtaatctgtc agcagtagct gcgccagtgg catttggttt tccggcactt
      601 tccactccag gagctggcgg tctggcggtc agtatttcag cgggagcatt atcggcagct
      661 attgctgata ttatggctgc cctgaaagga ccgtttaaat ttggtctttg gggggtggct
      721 ttatatggtg tattgccatc acaaatagcg aaagatgacc ccaatatgat gtcaaagatt
      781 gtgacgtcat tacccgcaga tgatattact gaatcacctg tcagttcatt acctctcgat
      841 aaggcaacag taaacgtaaa tgttcgtgtt gttgatgatg taaaagacga gcgacagaat
      901 atttcggttg tttcaggtgt tccgatgagt gttccggtgg ttgatgcaaa acctaccgaa
      961 cgtccgggtg tttttacggc atcaattcca ggtgcacctg ttctgaatat ttcagttaat
     1021 aacagtacgc cagcagtaca gacattaagc ccaggtgtta caaataatac tgataaggat
     1081 gttcgcccgg caggatttac tcagggtggt aataccaggg atgcagttat tcgattcccg
     1141 aaggacagcg gtcataatgc cgtatatgtt tcagtgagtg atgttcttag ccctgaccag
     1201 gtaaaacaac gtcaagatga agaaaatcgc cgtcagcagg aatgggatgc tacgcatccg
     1261 gttgaagcgg ctgagcgaaa ttatgaacgc gcgcgtgcag agctgaatca ggcaaatgaa
     1321 gatgttgcca gaaatcagga gcgacaggct aaagctgttc aggtttataa ttcgcgtaaa
     1381 agcgaacttg atgcagcgaa taaaactctt gctgatgcaa tagctgaaat aaaacaattt
     1441 aatcgatttg cccatgaccc aatggctggc ggtcacagaa tgtggcaaat ggccgggctt
     1501 aaagcccagc gggcgcagac ggatgtaaat aataagcagg ctgcatttga tgctgctgca
     1561 aaagagaagt cagatgctga tgctgcattg agttctgcta tggaaagcag gaagaagaaa
     1621 gaagataaga aaaggagtgc tgaaaataat ttaaacgatg aaaagaataa gcccagaaaa
     1681 ggttttaaag attacgggca tgattatcat ccagctccga aaactgagaa tattaaaggg
     1741 cttggtgatc ttaagcctgg gataccaaaa acaccaaagc agaatggtgg tggaaaacgc
     1801 aagcgctgga ctggagataa agggcgtaag atttatgagt gggattctca gcatggtgag
     1861 cttgaggggt atcgtgccag tgatggtcag catcttggct catttgaccc taaaacaggc
     1921 aatcagttga aaggtccaga tccgaaacga aatatcaaga aatatctttg agaggaagtt
     1981 atgggactta aattggattt aacttggttt gataaaagta cagaagattt taagggtgag
     2041 gagtattcaa aagattttgg agatgacggt tcagttatgg aaagtctagg tgtgcctttt
     2101 aaggataatg ttaataacgg ttgctttgat gttatagctg aatgggtacc tttgctacaa
     2161 ccatacttta atcatcaaat tgatatttcc gataatgagt attttgtttc gtttgattat
     2221 cgtgatggtg attggtgatc aaatattatc agggatgagt tgatatacgg gcttctagtg
     2281 ttcatggatg aacgctggag cctccaaatg tagaaatgtt atatttttta ttgagttctt
     2341 ggttataatt gctccgcaat gatttaaata agcattattt aaaacattct caggagaggt
     2401 gaaggtggag ctaaaaaaaa gtattggtga ttacactgaa accgaattca aaaaatttat
     2461 tgaagacatc atcaattgtg aaggtgatga aaaaaaacag gatgataacc tcgagtattt
     2521 tataaatgtt actgagcatc ctagtggttc tgatctgatt tattacccag aaggtaataa
     2581 tgatggtagc cctgaaggtg ttattaaaga gattaaagaa tggcgagccg ctaacggtaa
     2641 gtcaggattt aaacagggct gaaatatgaa tgccggttgt ttatggatga atggctggca
     2701 ttctttcaca acaaggagtc gttatgaaaa aaataacagg gattatttta ttgcttcttg
     2761 cagtcattat tctgtctgca tgtcaggcaa actatatccg ggatgttcag ggcgggaccg
     2821 tatctccgtc atcaacagct gaagtgaccg gattagcaac gcagtaaccc gaaatcctct
     2881 ttgacaaaaa caaagcgtgt caggctgatt ctgatgcgct ttttttttga aatgtcacaa
     2941 aaattccatg tgggagatgg
//