LOCUS       BBa_K1314004            1081 bp    DNA     linear       01-Jun-2024
DEFINITION  Ptet mRFP with random sequence
ACCESSION   BBa_K1314004
VERSION     BBa_K1314004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..150
                     /label=random sequence/spacer
     misc_feature    1..150
                     /label=BBa_K1314002
     misc_feature    159..177
                     /label=TetR 1
     misc_feature    159..212
                     /label=BBa_R0040
     promoter        178..183
                     /label=-35
     misc_feature    184..202
                     /label=TetR 2
     promoter        201..206
                     /label=-10
     promoter        159..212
                     /label=BBa_R0040
     misc_feature    225..228
                     /label=conserved
     RBS             221..232
                     /label=BBa_B0034
     CDS             239..913
                     /label=mrfp1
     misc_feature    920..944
                     /label=Help:Barcodes
     CDS             239..919
                     /label=BBa_E1010
     stem_loop       964..1007
                     /label=stem_loop
     misc_feature    953..1032
                     /label=BBa_B0010
     terminator      953..1032
                     /label=BBa_B0010
     stem_loop       1048..1067
                     /label=T7 TE
     misc_feature    1074..1074
                     /label=stop
     polyA_site      1068..1081
                     /label=polya
     misc_feature    1041..1081
                     /label=BBa_B0012
     terminator      1041..1081
                     /label=BBa_B0012
     misc_feature    159..1081
                     /label=BBa_I13521
ORIGIN
        1 ggaattgggg cccttgtggc ctaaatccaa ccgcggatca gtcagggcgc ggatcccctg
       61 caacgtcgcg acgctagaga tactctatcc agcggtagct cgtgtggttc aaagggtaag
      121 ccgtcacgct ggcttcggag gtctatctgc tactagagtc cctatcagtg atagagattg
      181 acatccctat cagtgataga gatactgagc actactagag aaagaggaga aatactagat
      241 ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc
      301 cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac
      361 ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct
      421 gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga
      481 ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga
      541 cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa
      601 agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat
      661 gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat
      721 caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta
      781 catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat
      841 cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca
      901 ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat
      961 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1021 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1081 a
//