LOCUS       BBa_K1314006            3040 bp    DNA     linear       19-May-2024
DEFINITION  nifH promoter+B0031+T7 RNA polymerase+ B0015
ACCESSION   BBa_K1314006
VERSION     BBa_K1314006.1  
FEATURES             Location/Qualifiers
     promoter        1..220
                     /label=nifH promoter
     promoter        1..220
                     /label=BBa_K1314000
     misc_feature    235..238
                     /label=conserved
     RBS             229..242
                     /label=BBa_B0031
     misc_feature    249..251
                     /label=start
     misc_feature    2898..2900
                     /label=stop
     misc_feature    2901..2903
                     /label=stop
     CDS             252..2897
                     /label=protein
     CDS             249..2903
                     /label=BBa_K145001
     stem_loop       2923..2966
                     /label=stem_loop
     misc_feature    2912..2991
                     /label=BBa_B0010
     terminator      2912..2991
                     /label=BBa_B0010
     stem_loop       3007..3026
                     /label=T7 TE
     misc_feature    3033..3033
                     /label=stop
     polyA_site      3027..3040
                     /label=polya
     misc_feature    3000..3040
                     /label=BBa_B0012
     terminator      3000..3040
                     /label=BBa_B0012
     terminator      2912..3040
                     /label=BBa_B0015
ORIGIN
        1 tgtcagtttt gtcacagggg gccggaccag gatggtggac gctcgatggg gatgtcgggc
       61 cattgttcgg ttgtagcaat tacaacagtc ggagtagggg gattgtaggg ggattgttgt
      121 gtatcagacc gccctgctgc tcccgtcgat ggataattaa tcatttaaaa tcaatggttt
      181 atttatgtgt tgcgggtgct ggcacagacg ctgcattacc tactagagtc acacaggaaa
      241 cctactagat gaacacgatt aacatcgcta agaacgactt ctctgacatc gaactggctg
      301 ctatcccgtt caacactctg gctgaccatt acggtgagcg tttagctcgc gaacagttgg
      361 cccttgagca tgagtcttac gagatgggtg aagcacgctt ccgcaagatg tttgagcgtc
      421 aacttaaagc tggtgaggtt gcggataacg ctgccgccaa gcctctcatc actaccctac
      481 tccctaagat gattgcacgc atcaacgact ggtttgagga agtgaaagct aagcgcggca
      541 agcgcccgac agccttccag ttcctgcaag aaatcaagcc ggaagccgta gcgtacatca
      601 ccattaagac cactctggct tgcctaacca gtgctgacaa tacaaccgtt caggctgtag
      661 caagcgcaat cggtcgggcc attgaggacg aggctcgctt cggtcgtatc cgtgaccttg
      721 aagctaagca cttcaagaaa aacgttgagg aacaactcaa caagcgcgta gggcacgtct
      781 acaagaaagc atttatgcaa gttgtcgagg ctgacatgct ctctaagggt ctactcggtg
      841 gcgaggcgtg gtcttcgtgg cataaggaag actctattca tgtaggagta cgctgcatcg
      901 agatgctcat tgagtcaacc ggaatggtta gcttacaccg ccaaaatgct ggcgtagtag
      961 gtcaagactc tgagactatc gaactcgcac ctgaatacgc tgaggctatc gcaacccgtg
     1021 caggtgcgct ggctggcatc tctccgatgt tccaaccttg cgtagttcct cctaagccgt
     1081 ggactggcat tactggtggt ggctattggg ctaacggtcg tcgtcctctg gcgctggtgc
     1141 gtactcacag taagaaagca ctgatgcgct acgaagacgt ttacatgcct gaggtgtaca
     1201 aagcgattaa cattgcgcaa aacaccgcat ggaaaatcaa caagaaagtc ctagcggtcg
     1261 ccaacgtaat caccaagtgg aagcattgtc cggtcgagga catccctgcg attgagcgtg
     1321 aagaactccc gatgaaaccg gaagacatcg acatgaatcc tgaggctctc accgcgtgga
     1381 aacgtgctgc cgctgctgtg taccgcaagg acaaggctcg caagtctcgc cgtatcagcc
     1441 ttgagttcat gcttgagcaa gccaataagt ttgctaacca taaggccatc tggttccctt
     1501 acaacatgga ctggcgcggt cgtgtttacg ctgtgtcaat gttcaacccg caaggtaacg
     1561 atatgaccaa aggactgctt acgctggcga aaggtaaacc aatcggtaag gaaggttact
     1621 actggctgaa aatccacggt gcaaactgtg cgggtgtcga taaggttccg ttccctgagc
     1681 gcatcaagtt cattgaggaa aaccacgaga acatcatggc ttgcgctaag tctccactgg
     1741 agaacacttg gtgggctgag caagattctc cgttctgctt ccttgcgttc tgctttgagt
     1801 acgctggggt acagcaccac ggcctgagct ataactgctc ccttccgctg gcgtttgacg
     1861 ggtcttgctc tggcatccag cacttctccg cgatgctccg agatgaggta ggtggtcgcg
     1921 cggttaactt gcttcctagt gaaaccgttc aggacatcta cgggattgtt gctaagaaag
     1981 tcaacgagat tctacaagca gacgcaatca atgggaccga taacgaagta gttaccgtga
     2041 ccgatgagaa cactggtgaa atctctgaga aagtcaagct gggcactaag gcactggctg
     2101 gtcaatggct ggcttacggt gttactcgca gtgtgactaa gcgttcagtc atgacgctgg
     2161 cttacgggtc caaagagttc ggcttccgtc aacaagtgct ggaagatacc attcagccag
     2221 ctattgattc cggcaagggt ctgatgttca ctcagccgaa tcaggctgct ggatacatgg
     2281 ctaagctgat ttgggaatct gtgagcgtga cggtggtagc tgcggttgaa gcaatgaact
     2341 ggcttaagtc tgctgctaag ctgctggctg ctgaggtcaa agataagaag actggagaga
     2401 ttcttcgcaa gcgttgcgct gtgcattggg taactcctga tggtttccct gtgtggcagg
     2461 aatacaagaa gcctattcag acgcgcttga acctgatgtt cctcggtcag ttccgcttac
     2521 agcctaccat taacaccaac aaagatagcg agattgatgc acacaaacag gagtctggta
     2581 tcgctcctaa ctttgtacac agccaagacg gtagccacct tcgtaagact gtagtgtggg
     2641 cacacgagaa gtacggaatc gaatcttttg cactgattca cgactccttc ggtaccattc
     2701 cggctgacgc tgcgaacctg ttcaaagcag tgcgcgaaac tatggttgac acatatgagt
     2761 cttgtgatgt actggctgat ttctacgacc agttcgctga ccagttgcac gagtctcaat
     2821 tggacaaaat gccagcactt ccggctaaag gtaacttgaa cctccgtgac atcttagagt
     2881 cggacttcgc gttcgcgtaa taatactaga gccaggcatc aaataaaacg aaaggctcag
     2941 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     3001 cacactggct caccttcggg tgggcctttc tgcgtttata
//