LOCUS BBa_K1314008 4129 bp DNA linear 19-May-2024 DEFINITION nifH promoter+B0031+T7 RNA polymerase+ B0015+ random sequence+ptet mRFP reporter ACCESSION BBa_K1314008 VERSION BBa_K1314008.1 FEATURES Location/Qualifiers promoter 1..220 /label=nifH promoter promoter 1..220 /label=BBa_K1314000 misc_feature 235..238 /label=conserved RBS 229..242 /label=BBa_B0031 misc_feature 249..251 /label=start misc_feature 2898..2900 /label=stop misc_feature 2901..2903 /label=stop CDS 252..2897 /label=protein CDS 249..2903 /label=BBa_K145001 stem_loop 2923..2966 /label=stem_loop misc_feature 2912..2991 /label=BBa_B0010 terminator 2912..2991 /label=BBa_B0010 stem_loop 3007..3026 /label=T7 TE misc_feature 3033..3033 /label=stop polyA_site 3027..3040 /label=polya misc_feature 3000..3040 /label=BBa_B0012 terminator 3000..3040 /label=BBa_B0012 terminator 2912..3040 /label=BBa_B0015 misc_feature 1..3040 /label=BBa_K1314006 misc_feature 3049..3198 /label=random sequence/spacer misc_feature 3049..3198 /label=BBa_K1314002 misc_feature 3207..3225 /label=TetR 1 misc_feature 3207..3260 /label=BBa_R0040 promoter 3226..3231 /label=-35 misc_feature 3232..3250 /label=TetR 2 promoter 3249..3254 /label=-10 promoter 3207..3260 /label=BBa_R0040 misc_feature 3273..3276 /label=conserved RBS 3269..3280 /label=BBa_B0034 CDS 3287..3961 /label=mrfp1 misc_feature 3968..3992 /label=Help:Barcodes CDS 3287..3967 /label=BBa_E1010 stem_loop 4012..4055 /label=stem_loop misc_feature 4001..4080 /label=BBa_B0010 terminator 4001..4080 /label=BBa_B0010 stem_loop 4096..4115 /label=T7 TE misc_feature 4122..4122 /label=stop polyA_site 4116..4129 /label=polya misc_feature 4089..4129 /label=BBa_B0012 terminator 4089..4129 /label=BBa_B0012 misc_feature 3207..4129 /label=BBa_I13521 misc_feature 3049..4129 /label=BBa_K1314004 ORIGIN 1 tgtcagtttt gtcacagggg gccggaccag gatggtggac gctcgatggg gatgtcgggc 61 cattgttcgg ttgtagcaat tacaacagtc ggagtagggg gattgtaggg ggattgttgt 121 gtatcagacc gccctgctgc tcccgtcgat ggataattaa tcatttaaaa tcaatggttt 181 atttatgtgt tgcgggtgct ggcacagacg ctgcattacc tactagagtc acacaggaaa 241 cctactagat gaacacgatt aacatcgcta agaacgactt ctctgacatc gaactggctg 301 ctatcccgtt caacactctg gctgaccatt acggtgagcg tttagctcgc gaacagttgg 361 cccttgagca tgagtcttac gagatgggtg aagcacgctt ccgcaagatg tttgagcgtc 421 aacttaaagc tggtgaggtt gcggataacg ctgccgccaa gcctctcatc actaccctac 481 tccctaagat gattgcacgc atcaacgact ggtttgagga agtgaaagct aagcgcggca 541 agcgcccgac agccttccag ttcctgcaag aaatcaagcc ggaagccgta gcgtacatca 601 ccattaagac cactctggct tgcctaacca gtgctgacaa tacaaccgtt caggctgtag 661 caagcgcaat cggtcgggcc attgaggacg aggctcgctt cggtcgtatc cgtgaccttg 721 aagctaagca cttcaagaaa aacgttgagg aacaactcaa caagcgcgta gggcacgtct 781 acaagaaagc atttatgcaa gttgtcgagg ctgacatgct ctctaagggt ctactcggtg 841 gcgaggcgtg gtcttcgtgg cataaggaag actctattca tgtaggagta cgctgcatcg 901 agatgctcat tgagtcaacc ggaatggtta gcttacaccg ccaaaatgct ggcgtagtag 961 gtcaagactc tgagactatc gaactcgcac ctgaatacgc tgaggctatc gcaacccgtg 1021 caggtgcgct ggctggcatc tctccgatgt tccaaccttg cgtagttcct cctaagccgt 1081 ggactggcat tactggtggt ggctattggg ctaacggtcg tcgtcctctg gcgctggtgc 1141 gtactcacag taagaaagca ctgatgcgct acgaagacgt ttacatgcct gaggtgtaca 1201 aagcgattaa cattgcgcaa aacaccgcat ggaaaatcaa caagaaagtc ctagcggtcg 1261 ccaacgtaat caccaagtgg aagcattgtc cggtcgagga catccctgcg attgagcgtg 1321 aagaactccc gatgaaaccg gaagacatcg acatgaatcc tgaggctctc accgcgtgga 1381 aacgtgctgc cgctgctgtg taccgcaagg acaaggctcg caagtctcgc cgtatcagcc 1441 ttgagttcat gcttgagcaa gccaataagt ttgctaacca taaggccatc tggttccctt 1501 acaacatgga ctggcgcggt cgtgtttacg ctgtgtcaat gttcaacccg caaggtaacg 1561 atatgaccaa aggactgctt acgctggcga aaggtaaacc aatcggtaag gaaggttact 1621 actggctgaa aatccacggt gcaaactgtg cgggtgtcga taaggttccg ttccctgagc 1681 gcatcaagtt cattgaggaa aaccacgaga acatcatggc ttgcgctaag tctccactgg 1741 agaacacttg gtgggctgag caagattctc cgttctgctt ccttgcgttc tgctttgagt 1801 acgctggggt acagcaccac ggcctgagct ataactgctc ccttccgctg gcgtttgacg 1861 ggtcttgctc tggcatccag cacttctccg cgatgctccg agatgaggta ggtggtcgcg 1921 cggttaactt gcttcctagt gaaaccgttc aggacatcta cgggattgtt gctaagaaag 1981 tcaacgagat tctacaagca gacgcaatca atgggaccga taacgaagta gttaccgtga 2041 ccgatgagaa cactggtgaa atctctgaga aagtcaagct gggcactaag gcactggctg 2101 gtcaatggct ggcttacggt gttactcgca gtgtgactaa gcgttcagtc atgacgctgg 2161 cttacgggtc caaagagttc ggcttccgtc aacaagtgct ggaagatacc attcagccag 2221 ctattgattc cggcaagggt ctgatgttca ctcagccgaa tcaggctgct ggatacatgg 2281 ctaagctgat ttgggaatct gtgagcgtga cggtggtagc tgcggttgaa gcaatgaact 2341 ggcttaagtc tgctgctaag ctgctggctg ctgaggtcaa agataagaag actggagaga 2401 ttcttcgcaa gcgttgcgct gtgcattggg taactcctga tggtttccct gtgtggcagg 2461 aatacaagaa gcctattcag acgcgcttga acctgatgtt cctcggtcag ttccgcttac 2521 agcctaccat taacaccaac aaagatagcg agattgatgc acacaaacag gagtctggta 2581 tcgctcctaa ctttgtacac agccaagacg gtagccacct tcgtaagact gtagtgtggg 2641 cacacgagaa gtacggaatc gaatcttttg cactgattca cgactccttc ggtaccattc 2701 cggctgacgc tgcgaacctg ttcaaagcag tgcgcgaaac tatggttgac acatatgagt 2761 cttgtgatgt actggctgat ttctacgacc agttcgctga ccagttgcac gagtctcaat 2821 tggacaaaat gccagcactt ccggctaaag gtaacttgaa cctccgtgac atcttagagt 2881 cggacttcgc gttcgcgtaa taatactaga gccaggcatc aaataaaacg aaaggctcag 2941 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 3001 cacactggct caccttcggg tgggcctttc tgcgtttata tactagaggg aattggggcc 3061 cttgtggcct aaatccaacc gcggatcagt cagggcgcgg atcccctgca acgtcgcgac 3121 gctagagata ctctatccag cggtagctcg tgtggttcaa agggtaagcc gtcacgctgg 3181 cttcggaggt ctatctgcta ctagagtccc tatcagtgat agagattgac atccctatca 3241 gtgatagaga tactgagcac tactagagaa agaggagaaa tactagatgg cttcctccga 3301 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca 3361 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa 3421 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt 3481 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact 3541 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt 3601 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg 3661 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc 3721 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct 3781 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa 3841 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa 3901 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc 3961 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga 4021 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 4081 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //