LOCUS BBa_K1314014 4313 bp DNA linear 27-May-2024 DEFINITION nifK-XhoI-nifH promoter T7 expression system(weak) ACCESSION BBa_K1314014 VERSION BBa_K1314014.1 FEATURES Location/Qualifiers misc_feature 1..170 /label=part of nifK misc_feature 171..176 /label=XhoI misc_feature 1..176 /label=BBa_K1314012 promoter 185..404 /label=nifH promoter promoter 185..404 /label=BBa_K1314000 misc_feature 419..422 /label=conserved RBS 413..426 /label=BBa_B0031 misc_feature 433..435 /label=start misc_feature 3082..3084 /label=stop misc_feature 3085..3087 /label=stop CDS 436..3081 /label=protein CDS 433..3087 /label=BBa_K145001 stem_loop 3107..3150 /label=stem_loop misc_feature 3096..3175 /label=BBa_B0010 terminator 3096..3175 /label=BBa_B0010 stem_loop 3191..3210 /label=T7 TE misc_feature 3217..3217 /label=stop polyA_site 3211..3224 /label=polya misc_feature 3184..3224 /label=BBa_B0012 terminator 3184..3224 /label=BBa_B0012 terminator 3096..3224 /label=BBa_B0015 misc_feature 185..3224 /label=BBa_K1314006 misc_feature 3233..3382 /label=random sequence/spacer misc_feature 3233..3382 /label=BBa_K1314002 misc_feature 3391..3409 /label=TetR 1 misc_feature 3391..3444 /label=BBa_R0040 promoter 3410..3415 /label=-35 misc_feature 3416..3434 /label=TetR 2 promoter 3433..3438 /label=-10 promoter 3391..3444 /label=BBa_R0040 misc_feature 3457..3460 /label=conserved RBS 3453..3464 /label=BBa_B0034 CDS 3471..4145 /label=mrfp1 misc_feature 4152..4176 /label=Help:Barcodes CDS 3471..4151 /label=BBa_E1010 stem_loop 4196..4239 /label=stem_loop misc_feature 4185..4264 /label=BBa_B0010 terminator 4185..4264 /label=BBa_B0010 stem_loop 4280..4299 /label=T7 TE misc_feature 4306..4306 /label=stop polyA_site 4300..4313 /label=polya misc_feature 4273..4313 /label=BBa_B0012 terminator 4273..4313 /label=BBa_B0012 misc_feature 3391..4313 /label=BBa_I13521 misc_feature 3233..4313 /label=BBa_K1314004 misc_feature 185..4313 /label=BBa_K1314008 ORIGIN 1 gtatcggctt cccgatcttc gaccgtcatc acctgcatcg ctccaccacc ctgggttacg 61 agggcgccat gcagatcctg accaccctgg tgaactcgat cctggaacgt ctggacgagg 121 aaacccgcgg tatgcaggcc accgactaca accacgacct ggtacgctaa ctcgagtact 181 agagtgtcag ttttgtcaca gggggccgga ccaggatggt ggacgctcga tggggatgtc 241 gggccattgt tcggttgtag caattacaac agtcggagta gggggattgt agggggattg 301 ttgtgtatca gaccgccctg ctgctcccgt cgatggataa ttaatcattt aaaatcaatg 361 gtttatttat gtgttgcggg tgctggcaca gacgctgcat tacctactag agtcacacag 421 gaaacctact agatgaacac gattaacatc gctaagaacg acttctctga catcgaactg 481 gctgctatcc cgttcaacac tctggctgac cattacggtg agcgtttagc tcgcgaacag 541 ttggcccttg agcatgagtc ttacgagatg ggtgaagcac gcttccgcaa gatgtttgag 601 cgtcaactta aagctggtga ggttgcggat aacgctgccg ccaagcctct catcactacc 661 ctactcccta agatgattgc acgcatcaac gactggtttg aggaagtgaa agctaagcgc 721 ggcaagcgcc cgacagcctt ccagttcctg caagaaatca agccggaagc cgtagcgtac 781 atcaccatta agaccactct ggcttgccta accagtgctg acaatacaac cgttcaggct 841 gtagcaagcg caatcggtcg ggccattgag gacgaggctc gcttcggtcg tatccgtgac 901 cttgaagcta agcacttcaa gaaaaacgtt gaggaacaac tcaacaagcg cgtagggcac 961 gtctacaaga aagcatttat gcaagttgtc gaggctgaca tgctctctaa gggtctactc 1021 ggtggcgagg cgtggtcttc gtggcataag gaagactcta ttcatgtagg agtacgctgc 1081 atcgagatgc tcattgagtc aaccggaatg gttagcttac accgccaaaa tgctggcgta 1141 gtaggtcaag actctgagac tatcgaactc gcacctgaat acgctgaggc tatcgcaacc 1201 cgtgcaggtg cgctggctgg catctctccg atgttccaac cttgcgtagt tcctcctaag 1261 ccgtggactg gcattactgg tggtggctat tgggctaacg gtcgtcgtcc tctggcgctg 1321 gtgcgtactc acagtaagaa agcactgatg cgctacgaag acgtttacat gcctgaggtg 1381 tacaaagcga ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa agtcctagcg 1441 gtcgccaacg taatcaccaa gtggaagcat tgtccggtcg aggacatccc tgcgattgag 1501 cgtgaagaac tcccgatgaa accggaagac atcgacatga atcctgaggc tctcaccgcg 1561 tggaaacgtg ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc tcgccgtatc 1621 agccttgagt tcatgcttga gcaagccaat aagtttgcta accataaggc catctggttc 1681 ccttacaaca tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa cccgcaaggt 1741 aacgatatga ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg taaggaaggt 1801 tactactggc tgaaaatcca cggtgcaaac tgtgcgggtg tcgataaggt tccgttccct 1861 gagcgcatca agttcattga ggaaaaccac gagaacatca tggcttgcgc taagtctcca 1921 ctggagaaca cttggtgggc tgagcaagat tctccgttct gcttccttgc gttctgcttt 1981 gagtacgctg gggtacagca ccacggcctg agctataact gctcccttcc gctggcgttt 2041 gacgggtctt gctctggcat ccagcacttc tccgcgatgc tccgagatga ggtaggtggt 2101 cgcgcggtta acttgcttcc tagtgaaacc gttcaggaca tctacgggat tgttgctaag 2161 aaagtcaacg agattctaca agcagacgca atcaatggga ccgataacga agtagttacc 2221 gtgaccgatg agaacactgg tgaaatctct gagaaagtca agctgggcac taaggcactg 2281 gctggtcaat ggctggctta cggtgttact cgcagtgtga ctaagcgttc agtcatgacg 2341 ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga taccattcag 2401 ccagctattg attccggcaa gggtctgatg ttcactcagc cgaatcaggc tgctggatac 2461 atggctaagc tgatttggga atctgtgagc gtgacggtgg tagctgcggt tgaagcaatg 2521 aactggctta agtctgctgc taagctgctg gctgctgagg tcaaagataa gaagactgga 2581 gagattcttc gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt ccctgtgtgg 2641 caggaataca agaagcctat tcagacgcgc ttgaacctga tgttcctcgg tcagttccgc 2701 ttacagccta ccattaacac caacaaagat agcgagattg atgcacacaa acaggagtct 2761 ggtatcgctc ctaactttgt acacagccaa gacggtagcc accttcgtaa gactgtagtg 2821 tgggcacacg agaagtacgg aatcgaatct tttgcactga ttcacgactc cttcggtacc 2881 attccggctg acgctgcgaa cctgttcaaa gcagtgcgcg aaactatggt tgacacatat 2941 gagtcttgtg atgtactggc tgatttctac gaccagttcg ctgaccagtt gcacgagtct 3001 caattggaca aaatgccagc acttccggct aaaggtaact tgaacctccg tgacatctta 3061 gagtcggact tcgcgttcgc gtaataatac tagagccagg catcaaataa aacgaaaggc 3121 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 3181 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agggaattgg 3241 ggcccttgtg gcctaaatcc aaccgcggat cagtcagggc gcggatcccc tgcaacgtcg 3301 cgacgctaga gatactctat ccagcggtag ctcgtgtggt tcaaagggta agccgtcacg 3361 ctggcttcgg aggtctatct gctactagag tccctatcag tgatagagat tgacatccct 3421 atcagtgata gagatactga gcactactag agaaagagga gaaatactag atggcttcct 3481 ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg 3541 gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg 3601 ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc 3661 agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga 3721 aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg 3781 ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac 3841 tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg 3901 aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc 3961 gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta 4021 aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc 4081 acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg 4141 gtgcttaata acgctgatag tgctagtgta gatcgctact agagccaggc atcaaataaa 4201 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 4261 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //