REACh2

BBa_K1319002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1319002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Osthege, Florian Gohr
Date created: 2014-09-29 11:00:00
Date modified: 2015-05-08 01:09:50

RFC[25]-compatible dark quencher based on K1319000 (E0030)



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K1319002_sequence (Version 1)

    Description

    This part is a RFC[25] dark quencher that is based upon K1319000 (the RFC[25] version of E0030/EYFP).

    Three mutations were introduced that eliminated fluorescence:
    • L90I
    • Y145W
    • H148R


    Notes

    Point mutations were introduced, starting from K1319000 (RFC25-YFP) as the template.

    Source

    The part is derived from K1319000.

    Sequence Annotation Location Component / Role(s)
    REACh2
    		1,711
    		feature/cds CDS

    igem#experience
    None
     
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1319002/1
     

    Attachments

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