BBa_K1319004

BBa_K1319004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1319004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Florian Gohr, Michael Osthege
Date created: 2014-09-24 11:00:00
Date modified: 2015-05-08 01:09:50

TEV protease with anti-self cleavage mutation S219V, codon optimized for E. coli



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K1319004_sequence (Version 1)

    Description

    This part is a TEV protease in RFC25 that was optimized for expression in E. coli. The part contains the S219V anti-self cleavage mutation.

    Notes

    The part does not contain start or stop codons, because it was designed for RFC25 compability

    Source

    The part was codon optimized using the genomic amino acid sequence, designed for RFC25 and mutated S219V for anti-self cleavage properties.

    Sequence Annotation Location Component / Role(s)
    TEV protease
    S219V
    		1,726
    655,657
    		CDS feature/cds
    sequence_alteration feature/mutation

    igem#experience
    None
     
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1319004/1
     

    Attachments

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