LOCUS       BBa_K132010              945 bp    DNA     linear       03-May-2024
DEFINITION  RBS+rhlR+terminator+Prhl
ACCESSION   BBa_K132010
VERSION     BBa_K132010.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..747
                     /label=BBa_C0171
     stem_loop       767..810
                     /label=stem_loop
     misc_feature    756..835
                     /label=BBa_B0010
     terminator      756..835
                     /label=BBa_B0010
     stem_loop       851..870
                     /label=T7 TE
     misc_feature    877..877
                     /label=stop
     polyA_site      871..884
                     /label=polya
     misc_feature    844..884
                     /label=BBa_B0012
     terminator      844..884
                     /label=BBa_B0012
     misc_feature    893..912
                     /label=RhlR
     promoter        911..916
                     /label=-35
     promoter        934..939
                     /label=-10
     misc_feature    945..945
                     /label=start
     promoter        893..945
                     /label=BBa_R0071
ORIGIN
        1 aaagaggaga aatactagat gaggaatgac ggaggctttt tgctgtggtg ggacggtttg
       61 cgtagcgaga tgcagccgat ccacgacagc cagggcgtgt tcgccgtcct ggaaaaggaa
      121 gtgcggcgcc tgggcttcga ttactacgcc tatggcgtgc gccacacgat tcccttcacc
      181 cggccgaaga ccgaggtcca tggcacctat cccaaggcct ggctggagcg ataccagatg
      241 cagaactacg gggccgtgga tccggcgatc ctcaacggcc tgcgctcctc ggaaatggtg
      301 gtctggagcg acagcctgtt cgaccagagc cggatgctct ggaacgaggc tcgcgattgg
      361 ggcctctgtg tcggcgcgac cttgccgatc cgcgcgccga acaatttgct cagcgtgctt
      421 tccgtggcgc gcgaccagca gaacatctcc agcttcgagc gcgaggaaat ccgcctgcgg
      481 ctgcgttgca tgatcgagtt gctgacccag aagctgaccg acctggagca tccgatgctg
      541 atgtccaacc cggtctgcct gagccatcgc gaacgcgaga tcctgcaatg gaccgccgac
      601 ggcaagagtt ccggggaaat cgccatcatc ctgagcatct ccgagagcac ggtgaacttc
      661 caccacaaga acatccagaa gaagttcgac gcgccgaaca agacgctggc tgccgcctac
      721 gccgcggcgc tgggtctcat ctaataatac tagagccagg catcaaataa aacgaaaggc
      781 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
      841 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agtcctgtga
      901 aatctggcag ttaccgttag ctttcgaatt ggctaaaaag tgttc
//