LOCUS       BBa_K132013             2152 bp    DNA     linear       08-May-2024
DEFINITION  Plac+rfp+Ptet+luxR+PluxPR
ACCESSION   BBa_K132013
VERSION     BBa_K132013.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..901
                     /label=mrfp1
     misc_feature    941..946
                     /label=Stop
     misc_feature    902..940
                     /label=LVA
     misc_feature    227..229
                     /label=Start
     misc_feature    227..946
                     /label=BBa_J04051
     stem_loop       966..1009
                     /label=stem_loop
     misc_feature    955..1034
                     /label=BBa_B0010
     terminator      955..1034
                     /label=BBa_B0010
     stem_loop       1050..1069
                     /label=T7 TE
     misc_feature    1076..1076
                     /label=stop
     polyA_site      1070..1083
                     /label=polya
     misc_feature    1043..1083
                     /label=BBa_B0012
     terminator      1043..1083
                     /label=BBa_B0012
     misc_feature    1..1083
                     /label=BBa_J04451
     misc_feature    1092..1110
                     /label=TetR 1
     misc_feature    1092..1145
                     /label=BBa_R0040
     promoter        1111..1116
                     /label=-35
     misc_feature    1117..1135
                     /label=TetR 2
     promoter        1134..1139
                     /label=-10
     promoter        1092..1145
                     /label=BBa_R0040
     misc_feature    1158..1161
                     /label=conserved
     RBS             1154..1165
                     /label=BBa_B0034
     misc_feature    1172..1173
                     /label=prefix
     misc_feature    1345..1345
                     /label=T
     misc_feature    1663..1663
                     /label=A
     CDS             1172..1921
                     /label=luxR
     misc_feature    1172..1927
                     /label=BBa_C0062
     misc_feature    1928..1952
                     /label=Help:Barcodes
     CDS             1172..1927
                     /label=BBa_C0062
     stem_loop       1972..2015
                     /label=stem_loop
     misc_feature    1961..2040
                     /label=BBa_B0010
     terminator      1961..2040
                     /label=BBa_B0010
     stem_loop       2056..2075
                     /label=T7 TE
     misc_feature    2082..2082
                     /label=stop
     polyA_site      2076..2089
                     /label=polya
     misc_feature    2049..2089
                     /label=BBa_B0012
     terminator      2049..2089
                     /label=BBa_B0012
     terminator      1961..2089
                     /label=BBa_B0015
     misc_feature    2098..2117
                     /label=LuxR/HSL
     promoter        2117..2122
                     /label=-35
     promoter        2139..2144
                     /label=-10
     misc_feature    2150..2150
                     /label=start
     misc_feature    2098..2152
                     /label=BBa_R0062
     promoter        2098..2152
                     /label=BBa_R0062
     misc_feature    1092..2152
                     /label=BBa_F2620
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 taggcctgct gcaaacgacg aaaactacgc tttagtagct taataatact agagccaggc
      961 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1021 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1081 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg
     1141 agcactacta gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata
     1201 cagaataatt aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc
     1261 tgatatgact aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc
     1321 tatggttaaa tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta
     1381 tgatgacgct aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc
     1441 accaattaat tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat
     1501 taaagaagcg aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa
     1561 caatggcttc ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt
     1621 atttttacat gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg
     1681 aaaaataaat atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg
     1741 tttagcgtgg gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag
     1801 tgagcgtact gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg
     1861 ctgccaaagt atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa
     1921 ttaataacac tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga
     1981 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2041 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagacc
     2101 tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aa
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