LOCUS       BBa_K132015             2663 bp    DNA     linear       04-May-2024
DEFINITION  Ptet+cfp+Ptet+rhlR+Prhl+luxI
ACCESSION   BBa_K132015
VERSION     BBa_K132015.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..842
                     /label=CFP (LVA)
     misc_feature    799..836
                     /label=SsrA
     misc_feature    837..842
                     /label=2
     misc_feature    149..149
                     /label=A
     misc_feature    81..842
                     /label=BBa_E0022
     misc_feature    81..842
                     /label=BBa_E0022
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     misc_feature    988..1006
                     /label=TetR 1
     misc_feature    988..1041
                     /label=BBa_R0040
     promoter        1007..1012
                     /label=-35
     misc_feature    1013..1031
                     /label=TetR 2
     promoter        1030..1035
                     /label=-10
     promoter        988..1041
                     /label=BBa_R0040
     misc_feature    1..1041
                     /label=BBa_K132009
     misc_feature    1054..1057
                     /label=conserved
     RBS             1050..1061
                     /label=BBa_B0034
     CDS             1068..1796
                     /label=BBa_C0171
     stem_loop       1816..1859
                     /label=stem_loop
     misc_feature    1805..1884
                     /label=BBa_B0010
     terminator      1805..1884
                     /label=BBa_B0010
     stem_loop       1900..1919
                     /label=T7 TE
     misc_feature    1926..1926
                     /label=stop
     polyA_site      1920..1933
                     /label=polya
     misc_feature    1893..1933
                     /label=BBa_B0012
     terminator      1893..1933
                     /label=BBa_B0012
     misc_feature    1942..1961
                     /label=RhlR
     promoter        1960..1965
                     /label=-35
     promoter        1983..1988
                     /label=-10
     misc_feature    1994..1994
                     /label=start
     promoter        1942..1994
                     /label=BBa_R0071
     misc_feature    1050..1994
                     /label=BBa_K132010
     misc_feature    2007..2010
                     /label=conserved
     RBS             2003..2014
                     /label=BBa_B0034
     misc_feature    2600..2631
                     /label=LVA
     CDS             2021..2599
                     /label=luxI
     misc_feature    2021..2638
                     /label=BBa_C0061
     misc_feature    2639..2663
                     /label=Help:Barcodes
     CDS             2021..2638
                     /label=BBa_C0061
     misc_feature    2003..2663
                     /label=BBa_C0261
     misc_feature    1050..2663
                     /label=BBa_K132011
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc
      121 ccatcctggt cgagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg
      181 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      241 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc
      301 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      361 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      421 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      481 acggcaacat cctggggcac aagctggagt acaactacat cagccacaac gtctatatca
      541 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
      601 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      661 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
      721 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      781 tggacgagct gtacaagagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat
      841 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc
     1021 agtgatagag atactgagca ctactagaga aagaggagaa atactagatg aggaatgacg
     1081 gaggcttttt gctgtggtgg gacggtttgc gtagcgagat gcagccgatc cacgacagcc
     1141 agggcgtgtt cgccgtcctg gaaaaggaag tgcggcgcct gggcttcgat tactacgcct
     1201 atggcgtgcg ccacacgatt cccttcaccc ggccgaagac cgaggtccat ggcacctatc
     1261 ccaaggcctg gctggagcga taccagatgc agaactacgg ggccgtggat ccggcgatcc
     1321 tcaacggcct gcgctcctcg gaaatggtgg tctggagcga cagcctgttc gaccagagcc
     1381 ggatgctctg gaacgaggct cgcgattggg gcctctgtgt cggcgcgacc ttgccgatcc
     1441 gcgcgccgaa caatttgctc agcgtgcttt ccgtggcgcg cgaccagcag aacatctcca
     1501 gcttcgagcg cgaggaaatc cgcctgcggc tgcgttgcat gatcgagttg ctgacccaga
     1561 agctgaccga cctggagcat ccgatgctga tgtccaaccc ggtctgcctg agccatcgcg
     1621 aacgcgagat cctgcaatgg accgccgacg gcaagagttc cggggaaatc gccatcatcc
     1681 tgagcatctc cgagagcacg gtgaacttcc accacaagaa catccagaag aagttcgacg
     1741 cgccgaacaa gacgctggct gccgcctacg ccgcggcgct gggtctcatc taataatact
     1801 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1861 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1921 ttctgcgttt atatactaga gtcctgtgaa atctggcagt taccgttagc tttcgaattg
     1981 gctaaaaagt gttctactag agaaagagga gaaatactag atgactataa tgataaaaaa
     2041 atcggatttt ttggcaattc catcggagga gtataaaggt attctaagtc ttcgttatca
     2101 agtgtttaag caaagacttg agtgggactt agttgtagaa aataaccttg aatcagatga
     2161 gtatgataac tcaaatgcag aatatattta tgcttgtgat gatactgaaa atgtaagtgg
     2221 atgctggcgt ttattaccta caacaggtga ttatatgctg aaaagtgttt ttcctgaatt
     2281 gcttggtcaa cagagtgctc ccaaagatcc taatatagtc gaattaagtc gttttgctgt
     2341 aggtaaaaat agctcaaaga taaataactc tgctagtgaa attacaatga aactatttga
     2401 agctatatat aaacacgctg ttagtcaagg tattacagaa tatgtaacag taacatcaac
     2461 agcaatagag cgatttttaa agcgtattaa agttccttgt catcgtattg gagacaaaga
     2521 aattcatgta ttaggtgata ctaaatcggt tgtattgtct atgcctatta atgaacagtt
     2581 taaaaaagca gtcttaaatg ctgcaaacga cgaaaactac gctttagtag cttaataact
     2641 ctgatagtgc tagtgtagat ctc
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