LOCUS       BBa_K132027             2781 bp    DNA     linear       18-Apr-2024
DEFINITION  cre induced by iptg
ACCESSION   BBa_K132027
VERSION     BBa_K132027.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    1170..1208
                     /label=LVA
     CDS             81..1208
                     /label=lacI-LVA
     misc_feature    81..1208
                     /label=BBa_C0012
     misc_feature    1209..1233
                     /label=Help:Barcodes
     CDS             81..1208
                     /label=BBa_C0012
     stem_loop       1253..1296
                     /label=stem_loop
     misc_feature    1242..1321
                     /label=BBa_B0010
     terminator      1242..1321
                     /label=BBa_B0010
     stem_loop       1337..1356
                     /label=T7 TE
     misc_feature    1363..1363
                     /label=stop
     polyA_site      1357..1370
                     /label=polya
     misc_feature    1330..1370
                     /label=BBa_B0012
     terminator      1330..1370
                     /label=BBa_B0012
     misc_feature    63..1370
                     /label=BBa_P0412
     misc_feature    1..1370
                     /label=BBa_I722007
     CDS             1379..1466
                     /label=end of LacI coding region (inactive)
     misc_feature    1379..1578
                     /label=BBa_R0010
     misc_feature    1467..1504
                     /label=CAP binding site
     promoter        1515..1520
                     /label=-35
     promoter        1539..1544
                     /label=-10
     misc_feature    1544..1578
                     /label=LacI binding site
     misc_feature    1551..1551
                     /label=start
     promoter        1379..1578
                     /label=BBa_R0010
     RBS             1587..1601
                     /label=RBS-1\Strong
     RBS             1594..1598
                     /label=RBS
     misc_feature    1587..1601
                     /label=BBa_B0030
     RBS             1587..1601
                     /label=BBa_B0030
     CDS             1608..2644
                     /label=BBa_J61047
     misc_feature    1587..2644
                     /label=BBa_I718007
     stem_loop       2664..2707
                     /label=stem_loop
     misc_feature    2653..2732
                     /label=BBa_B0010
     terminator      2653..2732
                     /label=BBa_B0010
     stem_loop       2748..2767
                     /label=T7 TE
     misc_feature    2774..2774
                     /label=stop
     polyA_site      2768..2781
                     /label=polya
     misc_feature    2741..2781
                     /label=BBa_B0012
     terminator      2741..2781
                     /label=BBa_B0012
     terminator      2653..2781
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
      121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
      181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
      241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
      301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
      361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
      421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
      481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
      541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
      601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
      661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
      721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
      781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
      841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
      901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
      961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag
     1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg
     1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagca
     1381 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     1441 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     1501 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     1561 ggataacaat ttcacacata ctagagatta aagaggagaa atactagatg tccaatttac
     1621 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
     1681 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
     1741 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
     1801 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
     1861 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
     1921 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
     1981 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
     2041 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
     2101 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
     2161 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
     2221 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
     2281 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     2341 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     2401 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     2461 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     2521 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc atgaactata
     2581 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattaag
     2641 aatttactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     2701 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     2761 gtgggccttt ctgcgtttat a
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