LOCUS       BBa_K1321115             691 bp    DNA     linear       02-May-2024
DEFINITION  CBDcex fused to phytochelatin (PC) EC20 with LacI promoter
ACCESSION   BBa_K1321115
VERSION     BBa_K1321115.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    227..229
                     /label=start
     misc_feature    221..235
                     /label=BBa_K1319106
     CDS             236..562
                     /label=BBa_K1321003
     misc_feature    563..568
                     /label=Scar 25
     misc_feature    563..568
                     /label=BBa_B0105
     CDS             569..691
                     /label=Synthetic Phytochelatin EC(20)
     CDS             569..691
                     /label=BBa_K1321005
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ccggcggtcc
      241 ggccgggtgc caggtgctgt ggggcgtcaa ccagtggaac accggcttca ccgcgaacgt
      301 caccgtgaag aacacgtcct ccgctccggt agacggctgg acgctcacgt tcagcttccc
      361 gtccggccag caggtcaccc aggcgtggag ctcgacggtc acgcagtccg gctcggccgt
      421 gacggtccgc aacgccccgt ggaacggctc gatcccggcg ggcggcaccg cgcagttcgg
      481 cttcaacggc tcgcacacgg gcaccaacgc cgcgccgacg gcgttctcgc tcaacggcac
      541 gccctgcacg gtcggcggca gcaccggcga atgtgaatgt gagtgcgaat gcgagtgtga
      601 atgtgagtgt gagtgtgaat gtgaatgcga gtgtgagtgc gagtgcgaat gtgagtgtga
      661 atgcgagtgc gaatgcgaat gcgagtgcgg c
//