LOCUS BBa_K1321304 4497 bp DNA linear 03-May-2024 DEFINITION pSEVA321-BB with J23117-RFP ACCESSION BBa_K1321304 VERSION BBa_K1321304.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Biobrick suffix CDS 1175..1834 /label=Chloramphenicol acetyltransferase misc_feature 1858..2093 /label=OriT misc_feature 2152..3300 /label=Replication protein TrfA misc_feature 4476..4497 /label=Biobrick prefix misc_feature 1..35 /label=J23117 misc_feature 37..42 /label=SpeI misc_feature 44..55 /label=RBS B0034 CDS 62..742 /label=mRFP misc_feature 776..904 /label=Double terminator B0015 misc_feature 913..920 /label=NotI misc_feature 920..925 /label=PstI ORIGIN 1 ttgacagcta gctcagtcct agggattgtg ctagctacta gtgaaagagg agaaatacta 61 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg 121 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg 181 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat 241 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc 301 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga 361 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta 421 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac 481 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga 541 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac 601 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga 661 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg 721 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg 781 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 841 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 901 tatatactag aagcggccgc tgcagcttgg actcctgttg atagatccag taatgacctc 961 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga 1021 gaatccaggg gtccccaata attacgattt aaattggcga aaatgagacg ttgatcggca 1081 cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga 1141 gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac 1201 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc 1261 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa 1321 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc 1381 tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca 1441 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata 1501 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga 1561 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc 1621 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc 1681 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat 1741 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca 1801 acagtactgc gatgagtggc agggcggggc gtaatttgac ttttgtcctt ttccgctgca 1861 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga 1921 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag 1981 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct 2041 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc gtaggccggc 2101 cgcgatgcag gtggctgctg aacccccagc cggaactgac cccacaaggc cctagcgttt 2161 gcaatgcacc aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc 2221 ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca 2281 catgaggcgg aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc 2341 gaacatccgt cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta 2401 gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt 2461 tttcttgcca cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc 2521 aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc 2581 cttcgtgtaa taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt 2641 gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac 2701 cagttcgtca tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt 2761 gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt 2821 gaacagggca gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc 2881 aatatcgaac aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc 2941 ggcctgcttg gcttcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt 3001 ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg 3061 ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag 3121 ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg actatcgagc cgacggactg 3181 gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc 3241 ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat 3301 tcaccctcct tgcgggattg ccccggaatt aattccccgg atcgatccgt cgatcttgat 3361 cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc 3421 ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc 3481 gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt 3541 gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc 3601 tgcggactgg ctttctacgt ggctgccatt tttggggtga ggccgttcgc ggccgagggg 3661 cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc gggagggttc gagaaggggg 3721 ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt taaaaacaag 3781 gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg 3841 gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag 3901 gtgcgcccct catctgtcag cactctgccc ctcaagtgtc aaggatcgcg cccctcatct 3961 gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca 4021 catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca 4081 gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga 4141 gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc 4201 gaggtatcca caacgccggc ggccctacat ggctctgctg tagtgagtgg gttgcgctcc 4261 ggcagcggtc ctgatccccc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 4321 tacggcgcgc ccagctgtct agggcggcgg atttgtccta ctcaggagag cgttcaccga 4381 caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg 4441 cctttaatta aagcggataa caatttcaca caggagaatt cgcggccgct tctagag //