LOCUS BBa_K1321328 4562 bp DNA linear 17-May-2024 DEFINITION J-sRNA-331Bb ACCESSION BBa_K1321328 VERSION BBa_K1321328.1 FEATURES Location/Qualifiers promoter 1..37 /label=pLacIQ RBS 38..64 /label=RBS B0034 CDS 65..844 /label=LuxR repressor BBa_C0062 misc_feature 853..981 /label=B0015 terminator promoter 990..1041 /label=pLux BBa_R0062 misc_feature 1042..1065 /label=UGPase complementary sRNA misc_feature 1066..1150 /label=Hqf recruiting hairpin loop misc_feature 1157..1205 /label=Terminator P22 promoter 1214..1268 /label=pLux BBa_R0062 misc_feature 1275..1305 /label=Synthetic medium RBS CDS 1306..1614 /label=E. coli Hfq misc_feature 1621..1677 /label=Strong terminator P11 misc_feature 1678..1690 /label=Biobrick Suffix misc_feature 1691..4541 /label=pSEVA331 backbone misc_feature 4541..4562 /label=BioBrick prefix misc_feature 4556..4561 /label=XbaI RE site misc_feature 1207..1212 /label=SpeI RE site misc_feature 1269..1274 /label=NsiI RE site ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt 121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa 181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta 241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat 301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac 361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc 421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca 481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca 541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca 601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc 661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc 721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta 781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga 841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa 1021 aatggtttgt tatagtcgaa tggctttttt aaggggctta atcactttct gttgggccat 1081 tgcattgcca ctgattttcc aacatataaa aagacaagcc cgaacagtcg tccgggcttt 1141 ttttctcgag ctcgagccaa ttattgaagg ccgctaacgc ggcctttttt tgtttctggt 1201 ctccctacta gtaacctgta ggatcgtaca ggtttacgca agaaaatggt ttgttatagt 1261 cgaataaaat gcatgacacg caaatactac aaggaggaat tctatatggc taaggggcaa 1321 tctttacaag atccgttcct gaacgcactg cgtcgggaac gtgttccagt ttctatttat 1381 ttggtgaatg gtattaagct gcaagggcaa atcgagtctt ttgatcagtt cgtgatcctg 1441 ttgaaaaaca cggtcagcca gatggtttac aagcacgcga tttctactgt tgtcccgtct 1501 cgcccggttt ctcatcacag taacaacgcc ggtggcggta ccagcagtaa ctaccatcat 1561 ggtagcagcg cgcagaatac ttccgcgcaa caggacagcg aagaaaccga ataatactag 1621 ctcggtacca aattccagaa aagagacgct ttcgagcgtc ttttttcgtt ttggtccgcg 1681 gccgctgcag cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga 1741 tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc caggggtccc 1801 caataattac gatttaaatt ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa 1861 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc 1921 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc 1981 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa 2041 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa 2101 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaatttcg 2161 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt 2221 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg 2281 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt 2341 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac 2401 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg 2461 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc 2521 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga 2581 gtggcagggc ggggcgtaat ttgacttttg tccttttccg ctgcataacc ctgcttcggg 2641 gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc 2701 caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt 2761 gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg 2821 gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg ccggccctac cggcgcggca 2881 gcgttacccg tgtcggcggc tccaacggct cgccatcgtc cagaaaacac ggctcatcgg 2941 gcatcggcag gcgctgctgc ccgcgccgtt cccattcctc cgtttcggtc aaggctggca 3001 ggtctggttc catgcccgga atgccgggct ggctgggcgg ctcctcgccg gggccggtcg 3061 gtagttgctg ctcgcccgga tacagggtcg ggatgcggcg caggtcgcca tgccccaaca 3121 gcgattcgtc ctggtcgtcg tgatcaacca ccacggcggc actgaacacc gacaggcgca 3181 actggtcgcg gggctggccc cacgccacgc ggtcattgac cacgtaggcc gacacggtgc 3241 cggggccgtt gagcttcacg acggagatcc agcgctcggc caccaagtcc ttgactgcgt 3301 attggaccgt ccgcaaagaa cgtccgatga gcttggaaag tgtcttctgg ctgaccacca 3361 cggcgttctg gtggcccatc tgcgccacga ggtgatgcag cagcattgcc gccgtgggtt 3421 tcctcgcaat aagcccggcc cacgcctcat gcgctttgcg ttccgtttgc acccagtgac 3481 cgggcttgtt cttggcttga atgccgattt ctctggactg cgtggccatg cttatctcca 3541 tgcggtaggg gtgccgcacg gttgcggcac catgcgcaat cagctgcaac ttttcggcag 3601 cgcgacaaca attatgcgtt gcgtaaaagt ggcagtcaat tacagatttt ctttaaccta 3661 cgcaatgagc tattgcgggg ggtgccgcaa tgagctgttg cgtacccccc ttttttaagt 3721 tgttgatttt taagtctttc gcatttcgcc ctatatctag ttctttggtg cccaaagaag 3781 ggcacccctg cggggttccc ccacgccttc ggcgcggctc cccctccggc aaaaagtggc 3841 ccctccgggg cttgttgatc gactgcgcgg ccttcggcct tgcccaaggt ggcgctgccc 3901 ccttggaacc cccgcactcg ccgccgtgag gctcgggggg caggcgggcg ggcttcgccc 3961 ttcgactgcc cccactcgca taggcttggg tcgttccagg cgcgtcaagg ccaagccgct 4021 gcgcggtcgc tgcgcgagcc ttgacccgcc ttccacttgg tgtccaaccg gcaagcgaag 4081 cgcgcaggcc gcaggccgga ggcttttccc cagagaaaat taaaaaaatt gatggggcaa 4141 ggccgcaggc cgcgcagttg gagccggtgg gtatgtggtc gaaggctggg tagccggtgg 4201 gcaatccctg tggtcaagct cgtgggcagg cgcagcctgt ccatcagctt gtccagcagg 4261 gttgtccacg ggccgagcga agcgagccag ccggtggccg ctcgcggcca tcgtccacat 4321 atccacgggc tggcaaggga gcgcagcgac cgcgcagggc gaagcccgga gagcaagccc 4381 gtaggggggg cgcgcccagc tgtctagggc ggcggatttg tcctactcag gagagcgttc 4441 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt 4501 tgatgccttt aattaaagcg gataacaatt tcacacagga gaattcgcgg ccgcttctag 4561 ag //