| Types | DnaRegion
|
| Roles | promoter
Regulatory
|
| Sequences | BBa_K1321333_sequence (Version 1)
|
Description
This regulatory part is made up of the Arabinose-Inducible promoter, pBAD, and its transcriptional inhibitor/activator, AraC. AraC inhibits gene expression in the absence of Arabinose by binding to the operator units present within the AraC and pBAD promoter sequences, and producing a DNA loop that prevents the RNA polymerase and CAP from binding and initiating transcription. When Arabinose is supplied, this binds to the AraC dimer and releases it from the operator site found in the pBAD promoter, at the same time it enhances transcription by allowing binding to the enhancer sites upstream.
Source
This part was cloned out of existing templates (such as BBa_K325218, BBa_325219, all of which contain sfGFP downstream of the pBAD promoter)
