Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Laura de Arroyo Garcia
Date created: 2014-10-07 11:00:00
Date modified: 2015-05-08 01:09:53
pSB-AcsAB
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K1321334_sequence (Version 1) |
Description
This part is made up of the coding sequence of the A and B elements of the acs, cellulose-producing operon. AcsA and acsB are transcribed and translated in the form of a single polypeptide, and form the main two domains (regulatory and catalytic) of the Cellulose Synthase enzyme in Gluconacetobacter Xylinus.Notes
The Ribosomal binding site of AcsAB is already included within the sequence. Because we are transferring this element from Gluconacetobacter into simpler organisms, such as E.coli, the native RBS was replaced with a new RBS, that would guarantee strong translation whilst maintaining the native stoichiometry for the final operon system to operate successfully.Source
The AcsAB coding sequence was extracted from NCBI, codon optimised and edited accordingly for further gene synthesis and cloning into pSB1C3.| Sequence Annotation | Location | Component / Role(s) |
| RBS - AcsAB AcsAB Coding Sequence Double Stop Codon Start Codon | 28,51 68,4678 4679,4684 68,71 | ribosome_entry_site feature/rbs CDS feature/cds feature/stop stop_codon start_codon feature/start |