LOCUS       BBa_K1321358             825 bp    DNA     linear       29-May-2024
DEFINITION  CBDcenA with linker fused to NiBP driven by LacI
ACCESSION   BBa_K1321358
VERSION     BBa_K1321358.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    227..229
                     /label=start
     misc_feature    221..235
                     /label=BBa_K1319106
     CDS             554..643
                     /label=Linker: Pro-Thr box
     CDS             236..553
                     /label=CBDCenA
     CDS             236..643
                     /label=BBa_K1321339
     misc_feature    644..649
                     /label=Scar 25
     misc_feature    644..649
                     /label=BBa_B0105
     CDS             650..825
                     /label=Nickel Binding Protein
     CDS             650..825
                     /label=BBa_K1321009
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ccggcgcgcc
      241 gggttgccgt gttgactacg cggttaccaa ccagtggccg ggtggtttcg gtgcgaacgt
      301 taccatcacc aacctgggtg acccggtttc ttcttggaaa ctggactgga cctacaccgc
      361 gggtcagcgt atccagcagc tgtggaacgg taccgcgtct accaacggtg gtcaggtttc
      421 tgttacctct ctgccgtgga acggttctat cccgactggt ggtaccgcgt ctttcggttt
      481 caacggttct tgggcgggtt ctaacccgac cccggcgtct ttctctctga acggtaccac
      541 ctgcacgggt accccgacga cctcgccaac tcctacgccg accccaacca cgccgactcc
      601 tacaccgacg cccaccccaa cgcctactcc gacggtcacc cccaccggcg cacaccatga
      661 agaacaacac ggcggacacc accaccatca ccatcacaac accaccacca ctaccatggc
      721 ggcgaacacc accatcacca ccacagctct catcatgaag aaggttgttg tagcactagc
      781 gatagtcatc atcaagaaga aggttgctgc cacgggcatc acgag
//