LOCUS       BBa_K1321361             783 bp    DNA     linear       10-May-2024
DEFINITION  CBDcenA with linker fused to NiBP driven by LacI
ACCESSION   BBa_K1321361
VERSION     BBa_K1321361.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    227..229
                     /label=start
     misc_feature    221..235
                     /label=BBa_K1319106
     CDS             236..601
                     /label=dCBD
     CDS             236..307
                     /label=Linker
     misc_feature    308..421
                     /label=cbh2 CBD
     CDS             422..493
                     /label=Linker
     misc_feature    494..601
                     /label=cbh1 CBD
     CDS             236..601
                     /label=BBa_K1321340
     misc_feature    602..607
                     /label=Scar 25
     misc_feature    602..607
                     /label=BBa_B0105
     CDS             608..783
                     /label=Nickel Binding Protein
     CDS             608..783
                     /label=BBa_K1321009
     CDS             554..643
                     /label=Linker: Pro-Thr box
     CDS             236..553
                     /label=CBDCenA
     CDS             236..643
                     /label=BBa_K1321339
     misc_feature    644..649
                     /label=Scar 25
     misc_feature    644..649
                     /label=BBa_B0105
     CDS             650..825
                     /label=Nickel Binding Protein
     CDS             650..825
                     /label=BBa_K1321009
     CDS             236..783
                     /label=BBa_K1321351
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ccggccccgg
      241 cgccaatccg cccggtacta cgaccacttc gcgtccagca accacgaccg ggtcgagccc
      301 cggcccacag gcctgttctt ccgtctgggg tcagtgcggc ggacagaact ggtctggccc
      361 tacctgctgt gcttctggca gcacatgcgt ctactctaac gactactata gccagtgcct
      421 gccgggtgct aacccgccag gcactaccac tacctctcgc cccgctacca ctacaggctc
      481 tagcccggga cctacccaaa gccactacgg ccagtgcgga ggcattggct acagcggccc
      541 taccgtctgc gcctccggaa ccacctgcca agtcctgaac ccgtactact cccagtgtct
      601 taccggcgca caccatgaag aacaacacgg cggacaccac caccatcacc atcacaacac
      661 caccaccact accatggcgg cgaacaccac catcaccacc acagctctca tcatgaagaa
      721 ggttgttgta gcactagcga tagtcatcat caagaagaag gttgctgcca cgggcatcac
      781 gag
//