LOCUS       BBa_K1321363             717 bp    DNA     linear       14-May-2024
DEFINITION  NiBP fused to CBDclos driven by LacI
ACCESSION   BBa_K1321363
VERSION     BBa_K1321363.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    227..229
                     /label=start
     misc_feature    221..235
                     /label=BBa_K1319106
     CDS             236..411
                     /label=Nickel Binding Protein
     CDS             236..411
                     /label=BBa_K1321009
     misc_feature    412..417
                     /label=Scar 25
     misc_feature    412..417
                     /label=BBa_B0105
     CDS             418..717
                     /label=BBa_K1321002
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ccggcgcaca
      241 ccatgaagaa caacacggcg gacaccacca ccatcaccat cacaacacca ccaccactac
      301 catggcggcg aacaccacca tcaccaccac agctctcatc atgaagaagg ttgttgtagc
      361 actagcgata gtcatcatca agaagaaggt tgctgccacg ggcatcacga gaccggctca
      421 tcaatgtcag ttgaatttta caactctaac aaatcagcac aaacaaactc aattacacca
      481 ataatcaaaa ttactaacac gtctgacagt gatttaaatt taaatgacgt aaaagttaga
      541 tattattaca caagtgatgg tacacaagga caaactttct ggtgtgacca tgctggtgca
      601 ttattaggaa atagctatgt tgataacact agcaaagtga cagcaaactt cgttaaagaa
      661 acagcaagcc caacatcaac ctatgataca tatgttgaat ttggatttgc aggcagc
//