LOCUS       BBa_K1333300             946 bp    DNA     linear       15-May-2024
DEFINITION  I13453-Rose42-RFP
ACCESSION   BBa_K1333300
VERSION     BBa_K1333300.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     RBS             228..233
                     /label=SD
     misc_feature    212..217
                     /label=Biobrick-Scar-adaptation
     stem_loop       139..153
                     /label=Predicted Stem Loop
     stem_loop       154..174
                     /label=Predicted Stem Loop
     stem_loop       177..209
                     /label=Predicted Stem Loop
     stem_loop       210..234
                     /label=Predicted Stem Loop extending to startcodon
     misc_feature    139..234
                     /label=BBa_K115001
     CDS             241..915
                     /label=mrfp1
     misc_feature    922..946
                     /label=Help:Barcodes
     CDS             241..946
                     /label=BBa_E1010
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagaggc cgcgacaagc ggtccgggcg ccctaggggc ccggcggaga
      181 cgggcgccgg aggtgtccga cgcctgctcg tcaagttctt gctccttgga ggattactag
      241 atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt
      301 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt
      361 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc
      421 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg
      481 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa
      541 gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac
      601 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc
      661 atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa
      721 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc
      781 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac
      841 atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt
      901 cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgc
//